|Tested species reactivity||Human|
|Published species reactivity||Non-human primate, Human, Rhesus monkey|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS with BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.
The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Analyte Specific Reagent
Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype.
MHCD0430 was used in flow cytometry to study the dynamics of Ag-specific CD4(+) T cells during antiretroviral therapy.
|Riou C,Tanko RF,Soares AP,Masson L,Werner L,Garrett NJ,Samsunder N,Abdool Karim Q,Abdool Karim SS,Burgers WA||Journal of immunology (Baltimore, Md. : 1950) (195:2273)||2015|
Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults.
MHCD0430 was used in flow cytometry to investigate the primary inflammatory and regulatory T cell responses induced by BCG vaccination in adults.
|Boer MC,Prins C,van Meijgaarden KE,van Dissel JT,Ottenhoff TH,Joosten SA||Clinical and vaccine immunology : CVI (22:778)||2015|
Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1.
MHCD0430 was used in flow cytometry to elucidate regulation of γ-globin expression in F-cells.
|Grieco AJ,Billett HH,Green NS,Driscoll MC,Bouhassira EE||PloS one (10:null)||2015|
|Rhesus monkey||Not Cited||
Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques.
MHCD0430 was used in flow cytometry and western blot to investigate how chronic morphine exposure affects T cell numbers and functions.
|Cornwell WD,Lewis MG,Fan X,Rappaport J,Rogers TJ||Journal of neuroimmunology (265:43)||2013|
Detection of JC virus-specific immune responses in a novel humanized mouse model.
MHCD0430 was used in flow cytometry to develop a novel animal model to study JCV infection.
|Tan CS,Broge TA,Seung E,Vrbanac V,Viscidi R,Gordon J,Tager AM,Koralnik IJ||PloS one (8:null)||2013|
Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.
MHCD0430 was used in flow cytometry to examine the role of IL-17A-producing multifunctional T cells in the pathogenesis of toxic shock syndrome.
|McArthur MA,Sztein MB||Clinical immunology (Orlando, Fla.) (146:140)||2013|
Vitamin B6 and the immunity in kidney transplant recipients.
MHCD0430 was used in flow cytometry to explore the importance of vitamin B6 in the recipients of allogeneic kidney graft.
|Jankowska M,Marsza¿¿ M,D¿bska-¿lizie¿ A,Carrero JJ,Lindholm B,Czarnowski W,Rutkowski B,Trzonkowski P||Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation (23:57)||2013|
Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis.
MHCD0430 was used in flow cytometry to study the effect of EBV load in patients with rheumatoid arthritis.
|Lünemann JD,Frey O,Eidner T,Baier M,Roberts S,Sashihara J,Volkmer R,Cohen JI,Hein G,Kamradt T,Münz C||Journal of immunology (Baltimore, Md. : 1950) (181:991)||2008|
HIV-induced changes in T cell signaling pathways.
MHCD0430 was used in flow cytometry to examine the changes in signaling pathways of human CD4(+) and CD8(+) T cells from HIV-infected patients.
|Schweneker M,Favre D,Martin JN,Deeks SG,McCune JM||Journal of immunology (Baltimore, Md. : 1950) (180:6490)||2008|
|Non-human primate||Not Cited||
Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey).
MHCD0430 was used in flow cytometry to discuss available reagents for studying the neotropical primate squirrel monkey.
|Contamin H,Loizon S,Bourreau E,Michel JC,Garraud O,Mercereau-Puijalon O,Behr C||Journal of immunological methods (297:61)||2005|
CD4+CD25+ regulatory T cells do not significantly contribute to direct pathway hyporesponsiveness in stable renal transplant patients.
MHCD0430 was used in flow cytometry to examine the role of CD4(+)CD25(+) cells in regulating alloresponses in human transplant recipients.
|Game DS,Hernandez-Fuentes MP,Chaudhry AN,Lechler RI||Journal of the American Society of Nephrology : JASN (14:1652)||2003|
High expression of Fas ligand by synovial fluid-derived gamma delta T cells in Lyme arthritis.
MHCD0430 was used in flow cytometry to study gamma delta T cells in the synovial fluid of human Lyme arthritis.
|Roessner K,Wolfe J,Shi C,Sigal LH,Huber S,Budd RC||Journal of immunology (Baltimore, Md. : 1950) (170:2702)||2003|
Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry.
MHCD0430 was used in flow cytometry to assess cryptomonad-derived phycobiliproteins for their applicability to flow cytometry.
|Telford WG,Moss MW,Morseman JP,Allnutt FC||Cytometry (44:16)||2001|
Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.
MHCD0430 was used in flow cytometry to characterize the cells present in the cutaneous blister fluid patients with toxic epidermal necrolysis.
|Le Cleach L,Delaire S,Boumsell L,Bagot M,Bourgault-Villada I,Bensussan A,Roujeau JC||Clinical and experimental immunology (119:225)||2000|
Unique CD8+ T cell-rich lymphoid aggregates in human uterine endometrium.
MHCD0430 was used in immunocytochemistry to describe the cellular composition and organization of lymphoid aggregates in the stratum basalis of uterine endometrium.
|Yeaman GR,Guyre PM,Fanger MW,Collins JE,White HD,Rathbun W,Orndorff KA,Gonzalez J,Stern JE,Wira CR||Journal of leukocyte biology (61:427)||1997|
IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.
MHCD0430 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation
|Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ||Leukemia (29:415)||2015|
Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection.
MHCD0430 was used in flow cytometry and immunocytochemistry to test if Nef modifies the composition of exosomes released by T lymphocytes
|de Carvalho JV,de Castro RO,da Silva EZ,Silveira PP,da Silva-Januário ME,Arruda E,Jamur MC,Oliver C,Aguiar RS,daSilva LL||PloS one (9:null)||2014|