TOPO cloining kits

Whether you’re PCR cloning with Taq DNA polymerase or a proofreading enzyme, there is a TOPO cloning kit available to take you quickly and efficiently into your downstream application.

Select the correct TOPO Cloning Kit by application

To order, review available kits by application, cloning strategy (TA, blunt, directional), advantages, and catalog numbers.

General subcloning

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
TOPO TA Cloning KitTA
  • Get your clone with 95% efficiency 
  • Selection of competent cells for high speed, electroporation, or routine cloning
TOPO TA Cloning Kit for Subcloning without Competent Cells450641
Mach1-T1RK451020
TOP10K450001
TOP10F'K455001
TOP10 ElectrocompK456001
MAX Efficiency DH5α-T1 Phage-ResistantK452001
Zero Blunt TOPO PCR Cloning KitBlunt
  • Unique technology minimizes background 
  • Get your clone with 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convenient 
  • Selection of competent cells for high speed, electroporation, or routine cloning
Zero Blunt TOPO PCR Cloning Kit without Competent Cells450245
Mach1-T1RK283020
TOP10K280020
TOP10 ElectrocompK286020
MAX Efficiency DH5α-T1 Phage-ResistantK282020

Long PCR fragments

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
TOPO XL-2 Complete PCR Cloning KitBlunt
  • Complete kit offering high cloning efficiencies for single PCR fragments up to 13kb in length 
  • Includes Platinum SuperFi Green PCR MasterMix, for exceptional fidelity and specificity
OmniMAX 2 T1R Chemically Competent cellsK805020

In vitro transcription

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
TOPO TA Cloning Kit Dual PromoterTA
  • Get your clone with 95% efficiency
  • Dual T7 and SP6 priming sites for in vitro transcription
Mach1-T1RK461020
TOP10F'K460001
TOP10 ElectrocompK466001
MAX Efficiency DH5α -T1 Phage-ResistantK462001

Sequencing

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
TOPO TA Cloning Kit for Sequencing  (pCR 4 TOPO Vector)TA
  • Get your clone with 95% efficiency   
  • Sequence more insert, less vector
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convenient
TOPO TA Cloning Kit for Sequencing without Competent Cells450030
Mach1-T1RK453020
TOP10K457501
TOP10 ElectrocompK458001
MAX Efficiency DH5α -T1 Phage-ResistantK289520
Zero Blunt TOPO PCR Cloning Kit for Sequencing (pCR 4 Blunt TOPO Vector)Blunt
  • Unique technology minimizes background 
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
Mach1-T1RK283520
TOP10K287520
MAX Efficiency DH5α-T1 Phage-ResistantK289520
TOP10 ElectrocompK288020

Expression in E. coli

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
Champion pET100 Series Directional TOPO Expression Kits (pET Directional TOPO Vector)Directional
  • Up to 90% of clones in the correct orientation 
  • High-level protein expression using E. coli 
  • Convenient purification and detection tags
BL21 Star(DE3)K10001
K10101
K10201
K15101

Expression in mammalian cells

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
pcDNA 3.3 TOPO TA Cloning KitTA
  • 3–5 higher expression compared to to conventional CMV vectors 
  • Ideal for expression of native protein
TOP10K830001
pcDNA 3.2/V5/GW/D-TOPO Cloning KitDirectional
  • Greater than 90% of clones in correct orientation
  • CMV promoter delivers high-level expression in mammalian cells 
  • C-terminal V5 and 6xHis tags for convenient detection and purification 
  • Neomycin selection
TOP10K244020
ViraPower HiPerform Lentiviral FastTiter TOPO Expression Kits (pLenti 7.3 Vector)TA
  • Greater than 4X higher expression compared to the original system 
  • Efficient TOPO cloning
Stbl3K495000

Entry into Gateway Entry Vectors

ProductTOPO cloningAdvantagesOne Shot Competent cellsOrder now
pCR8/GW/TOPO TA Cloning KitTA
  • Includes plasmid miniprep kit 
  • Fast growing cells for fast miniprep
Mach1-T1RK532000
TA
  • Fast growing cells for fast miniprep
Mach1-T1RK252020
TOP10K250020
pENTR/TEV/DTOPO Cloning KitDirectional
  • Fast growing cells for fast miniprep
Mach1-T1RK252520
pENTR/D-TOPO Cloning KitDirectionalTOP10K240020
pENTR/SD/DTOPO Cloning KitDirectionalTOP10K242020

Resources

Cloning Support Center

Find tips, troubleshooting help, and resources for your cloning applications.

Cloning services

We offer everything from custom vector design to high-throughput protein expression.

Molecular Cloning Education

Learn about the foundations of recombinant DNA technology


Tools