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Whether you’re PCR cloning with Taq DNA polymerase or a proofreading enzyme, there is a TOPO® cloning kit available to take you quickly and efficiently into your downstream application.

 

Application of cloned PCR product Product Type of TOPO® cloning Advantages One Shot® Competent Cells Cat. no.  
General subcloning TOPO® TA Cloning® Kit TA
  • Get your clone 95% efficiency
  • Selection of competent cells for high speed, electroporation, or routine cloning
  • NEW

    TOPO® TA Cloning® Kit for Subcloning without Competent Cells

    450641 Order Now
    Mach1-T1R K451020 Order Now
    TOP10 K450001 Order Now
    TOP10F’ K455001 Order Now
    TOP10 Electrocomp™ K456001 Order Now
    MAX Efficiency® DH5α™-T1 Phage-Resistant K452001 Order Now
    Zero Blunt® TOPO® PCR Cloning Kit Blunt
  • Unique technology minimizes background
  • Get your clone 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convenient
  • Selection of competent cells for high speed, electroporation, or routine cloning
  • NEW

    Zero Blunt® TOPO® PCR Cloning Kit without Competent Cells

    450245 Order Now
    Mach1™-T1R K283020 Order Now
    TOP10 K280020 Order Now
    TOP10 Electrocomp™ K286020 Order Now
    MAX Efficiency® DH5α™-T1 Phage-Resistant K282020 Order Now
    Long PCR fragments TOPO® XL PCR Cloning Kit TA
  • Combining TOPO®, ZeroBackground™ and Crystal Violet technologies makes for easy cloning of large fragments 3-10 kb.
  • Unique technology to minimize background
  • Mach1™-T1R K703020 Order Now
    TOP10 K475010 Order Now
    TOP10 Electrocomp™ K470010 Order Now
    In vitro transcription TOPO® TA Cloning® Kit Dual Promoter TA
  • Get your clone 95% efficiency
  • Dual T7 and SP6 priming sites for in vitro transcription
  • Mach1™-T1R K461020 Order Now
    TOP10F’ K460001 Order Now
    TOP10 Electrocomp™ K466001 Order Now
    MAX Efficiency® DH5α™ -T1 Phage-Resistant K462001 Order Now
    Sequencing TOPO® TA Cloning® Kit for Sequencing  (pCR® 4 TOPO® Vector) TA
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
  • Multiple primer sites (T7, T3, M13F, M13R) makes sequence analysis easy and convenient 
  • NEW

    TOPO® TA Cloning® Kit for Sequencing without Competent Cells

    450030 Order Now
    Mach1™-T1R K453020 Order Now
    TOP10 K457501 Order Now
    TOP10 Electrocomp™ K458001 Order Now
    MAX Efficiency® DH5α™ -T1 Phage-Resistant K289520 Order Now
    Zero Blunt® TOPO® PCR Cloning Kit for Sequencing (pCR® 4 Blunt TOPO® Vector) Blunt
  • Unique technology minimizes background
  • Get your clone 95% efficiency
  • Sequence more insert, less vector
  • Mach1™-T1R K283520 Order Now
    TOP10 K287520 Order Now
    MAX Efficiency® DH5α™-T1 Phage-Resistant K289520 Order Now
    TOP10 Electrocomp™ K288020 Order Now
    Expression in E. coli Champion™ pET100 Series Directional TOPO® Expression Kits (pET Directional TOPO® Vector) Directional
  • Up to 90% of clones in the correct orientation
  • High-level protein expression using E. coli
  • Convenient purification and detection tags
  • BL21 Star™(DE3) K10001 Order Now
    K10101 Order Now
    K10201 Order Now
    K15101 Order Now
    Expression in mammalian cells pcDNA™ 3.3 TOPO® TA Cloning® Kit TA
  • 3–5 higher expression compared to conventional CMV vectors
  • Ideal for expression of native protein
  • TOP10 K830001 Order Now
    pcDNA™ 3.2/V5/GW/D-TOPO® Cloning Kit Directional
  • Greater than 90% of clones in correct orientation
  • CMV promoter delivers high-level expression in mammalian cells
  • C-terminal V5 and 6xHis tags for convenient detection and purification
  • Neomycin selection
  • TOP10 K244020 Order Now
    ViraPower™ HiPerform™ Lentiviral FastTiter™ TOPO® Expression Kits (pLenti 7.3 Vector) TA
  • Greater than 4X higher expression compared to the original system
  • Efficient TOPO® cloning
  • Stbl3™ K495000 Order Now
    Entry into Gateway® Entry Vectors pCR®8/GW/TOPO® TA Cloning® Kit TA
  • Includes Plasmid miniprep kit
  • Fast growing cells for fast miniprep
  • Mach1™-T1R K532000 Order Now
    TA
  • Fast growing cells for fast miniprep
  • Mach1™-T1R K252020 Order Now
        TOP10 K250020 Order Now
    pENTR™/TEV/DTOPO® Cloning Kit Directional
  • Fast growing cells for fast miniprep
  • Mach1™-T1R K252520 Order Now
    pENTR™/D-TOPO® Cloning Kit Directional   TOP10 K240020 Order Now
    pENTR™/SD/DTOPO® Cloning Kit Directional   TOP10 K242020 Order Now