Search
On this page:
Precision reagents for confident drug development and bioprocess analytics
Thermo Scientific CaptureSelect Conjugated Ligands offer high-specificity analytical performance for reliable quantitation and characterization of biologics—including monoclonal antibodies (mAbs), viral vectors, recombinant proteins, and other complex biotherapeutics.
Each ligand conjugate is engineered for consistent binding affinity and assay-ready convenience across ELISA, surface plasmon resonance (SPR), and bio-layer interferometry (BLI) platforms.
What are CaptureSelect conjugated ligands?
CaptureSelect conjugated ligands are single-domain antibody fragments (VHH) directionally coupled to functional reporters—such as biotin, horseradish peroxidase (HRP), or Alexa Fluor dyes. Built on Thermo Fisher Scientific’s proven CaptureSelect ligand technology, they help enable precise, target-specific recognition while maintaining native binding activity. Available in ready-to-use analytical formats, these conjugates help simplify assay setup and improve data consistency across methods and laboratories.
Why choose CaptureSelect conjugated ligands?
CaptureSelect Conjugated Ligands feature:
- Ready-to-use conjugates available in biotin, HRP, and Alexa Fluor formats
- High binding specificity for antibodies, proteins, and viral vectors
- Compatibility with several analytical platforms including ELISA, SPR, and BLI assay platforms
- Reproducible performance across R&D, process, and QC workflows
- Produced in yeast - animal origin free
CaptureSelect quantitation workflow
CaptureSelect conjugated ligands facilitate a straightforward, ELISA-like workflow—offering high specificity and reduced assay optimization. This simple, familiar format allows scientists to generate precise, reproducible results from early method development through QC.
What are the differences between Biotin, HRP, and Alexa Fluor conjugate formats?
Selecting the optimal format depends on assay design, throughput requirements and detection modality.
- Biotin conjugates are an ideal choice for immobilization on streptavidin surfaces in capture assays and kinetic platforms
- HRP (Horseradish Peroxidase) conjugates enable enzymatic signal amplification in detection assays (e.g., ELISA) for low-abundance analytes
- Alexa Fluor conjugates allow fluorescent labelling for multiplexed detection, flow-cytometry adaptation, or high-throughput screening
Applications of CaptureSelect conjugated ligands
CaptureSelect ligand conjugates are designed to enhance accuracy and efficiency across diverse analytical formats, including ELISA, SPR, BLI, Western blot, and magnetic bead capture workflows.
Antibody and mAb characterization
Quantify antibody subclasses, detect product-related impurities, and monitor Fab or Fc fragments with high-affinity ligands engineered to maintain specificity across complex sample matrices. CaptureSelect conjugates can simplify assay setup while supporting reproducible detection of traditional and engineered mAbs during development and release.
Protein and hormone analysis
Characterize recombinant proteins, hormones, and glycoproteins with consistent binding kinetics and low background. CaptureSelect conjugates offer a reliable platform for quantitation and comparability assessments in regulated analytical environments.
Viral vector analytics
Achieve sensitive, reproducible detection of adeno-associated virus (AAV), lentivirus, and other gene therapy vectors. CaptureSelect antibody conjugates help enable direct capture and quantitation across capsid types and serotypes, supporting viral vector quality assessment in bioprocessing and QC.
Exosome and extracellular vesicle detection
Enable sensitive capture and profiling of extracellular vesicles and exosomes to support biomarker discovery and therapeutic development. CaptureSelect conjugates allow for a consistent, high-affinity foundation for vesicle characterization across assay formats.
Frequently asked questions
Request a technical consultation
No matter your questions, we are here to help. Our experienced team of scientists and technical specialists is ready to assist you in choosing the right solution and products. Complete this form to start the conversation.
CaptureSelect ligand products
Ready-to-use CaptureSelect conjugated ligands are offered in 100 µg and 500 µg sizes to support scalability in your analytical workflow.
- Antibodies, fragments, and derivatives
- Proteins and hormones
- Viral vectors
- Exosomes
Target |
Conjugate |
Description |
|---|---|---|
Biotin |
Binds human IgG and all Fab fragments thereof, through recognition of the CH1 domain on all four human IgG subclasses. |
|
Biotin |
Binds human IgG, through recognition of the Fc region (CH3 domain) on all four human IgG subclasses. |
|
Biotin |
Binds IgG through recognition of the Fc region on IgG of multiple species. View product sheet for species coverage. |
|
Biotin |
Binds to rabbit IgG (immunoglobulin G) from recombinant and plasma sources. The ligand shows no cross-binding to bovine, horse, sheep, goat, mouse, rat, and human IgG. Moderate cross-binding was observed towards swine IgG. |
|
Biotin |
Binds human IgA, IgA-dimers and secretory IgA (sIgA), through recognition of the Fc region on both human IgA subclasses. |
|
Biotin |
Binds human, mouse, and rat IgM. |
|
Biotin |
Binds to human IgE (immunoglobulin E) from recombinant and plasma sources, through recognition of the Fc region (CH4 domain). |
|
Biotin |
Binds all human lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any human antibody, Fab fragment, or free light chain. |
|
Biotin |
Binds all human kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any human antibody, Fab fragment, or free light chain. |
|
Biotin |
Binds all mouse and rat kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any mouse and rat antibody, Fab fragment, or free light chain. |
|
Biotin |
Binds all mouse lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any mouse antibody, Fab fragment, or free light chain. |
|
Biotin |
Binds human IgG3 without any cross-binding to other human IgG subclasses or antibody isotypes. |
|
Biotin |
Binds human IgG4 without any cross-binding to other human IgG subclasses or antibody isotypes. |
|
Biotin |
High affinity capture of human IgG Fab fragments that possess a kappa light chain. Extreme low off-rates are achieved through avidity by targeting the CL-kappa domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics. |
|
Biotin |
High affinity capture of human IgG Fab fragments that possess a lambda light chain. Extreme low off-rates are achieved through avidity by targeting the CL-lambda domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics. |
|
Biotin |
Binds highly selective to free human kappa light chains and dimers thereof, through recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments, thereby providing a unique tool for specific detection and quantitation of free kappa light chains in (crude) antibody preps. |
|
Biotin |
Binds highly selective to human IgG, through recognition of the Fc region on all four human IgG subclasses without cross-binding to mouse, rat, rhesus, and cynomolgus monkey IgG, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays. |
Target |
Conjugate |
Description |
Biotin HRP Alexa Fluor 488 |
Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein |
|
Biotin |
Binds to human Factor X and its activated form Factor Xa from plasma and recombinant sources |
|
Biotin |
Binds recombinant as well as plasma derived human Factor IX (FIX) |
|
Biotin |
Binds recombinant (full length and B-domain deleted) as well as plasma derived human Factor VIII (FVIII), through recognition of the FVIII light chain |
|
Biotin |
Binds recombinant as well as plasma derived human Factor VII (FVII) |
|
Biotin |
Binds to the alpha chain of recombinant as well as natural sourced hCG (human chorionic gonadotropin), LH (luteinizing hormone), TSH (Thyroid-stimulating hormone) and FSH (follicle-stimulating hormone) |
|
Biotin |
Binds recombinant as well as natural sourced human serum albumin (HSA) including HSA-fusion proteins |
|
Biotin |
Binds recombinant as well as natural sourced human α1-Antitrypsin (AAT) |
|
Biotin |
Binds recombinant as well as natural sourced human C1-Inhibitor (C1-inh, C1 esterase inhibitor) |
|
Biotin |
Binds recombinant as well as natural sourced human Fibrinogen (coagulation factor I, FI) |
|
Biotin |
Binds recombinant as well as natural sourced human Transferrin |
|
Biotin |
Binds recombinant as well as natural sourced human Prothrombin (coagulation factor II, FII) |
|
Biotin |
Binds recombinant as well as natural sourced human Growth Hormone (hGH). |
|
Biotin |
Binds recombinant as well as natural sourced human Follicle Stimulating Hormone (FSH), through recognition of an epitope specific for intact FSH only |
|
Biotin |
Binds recombinant and natural-sourced human Erythropoietin (EPO) |
Target |
Conjugate |
Description |
Biotin
|
Binds with high affinity to adeno-associated virus particles of serotype 8 (AAV8). Note that it may cross-bind towards other AAV serotypes |
|
Biotin HRP Alexa Fluor 647 |
Binds with high affinity and selectivity to adeno-associated virus particles of serotype 9 (AAV9) |
|
Biotin HRP Alexa Fluor 647 |
Binds with high affinity and selectivity to adeno-associated viral (AAV) particles of a wide variety of serotypes |
|
Biotin |
Binds with high affinity and selectivity to recombinant hexon capsomeres of Adenovirus serotype 5 (AdV5) particles as well as free hexon proteins thereof |
Target |
Conjugate |
Description |
Biotin
|
Binds the tetraspanin molecule CD81 (Cluster of Differentiation 81; TAPA1 or TSPAN28) expressed on extracellular vesicles (EVs) like exosomes. |
|
Biotin
|
Binds the tetraspanin molecule CD63 (Cluster of Differentiation 63; LAMP3 or LIMP1) expressed on extracellular vesicles (EVs) like exosomes. |
|
Biotin
|
Binds the tetraspanin molecule CD9 (Cluster of Differentiation 9; MIC3, MRP1 or TSPAN29) expressed on extracellular vesicles (EVs) like exosomes. |
Analytics Knowledge Hub
Explore the Analytics Knowledge Hub and discover a robust collection of webinars, articles, eBooks, infographics, and other digital resources to help you optimize analytical testing in your bioprocess workflow.
Additional bioprocessing resources
Bioprocessing resources
For Research Use Only. Not for use in diagnostic procedures.