One Shot® MAX Efficiency® DH5α™-T1R Competent Cells

Catalog number: 12297016

Invitrogen™  Related applications: Transformation

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Description

One Shot® MAX Efficiency® DH5α™-T1R Competent Cells are derived from the popular DH5α™ strain. DH5α™-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. They offer added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic. In addition to phage resistance, DH5α™-T1R E. coli offer the following benefits:

• Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Reduced occurrence of unwanted recombination in cloned DNA (recA1)
• Efficient transformation of unmethylated DNA from PCR applications (hsdR)
• Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15)

Convenient and efficient format
DH5α™-T1R E. coli are supplied in the convenient, single-reaction One Shot® format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: DH5α™
Product Size: 20 x 50 µL
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): Yes
High Throughput Compatibility: Not High Throughput-Compatible (Manual)

Contents & storage

Contains:
• One Shot® MAX Efficiency® DH5α™-T1R Competent Cells: 20 vials, 50 µl each ( total of 1 ml)
• pUC19 DNA (10 pg/ul): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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