Invitrogen™

One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells

Catalog number: 12297016
Invitrogen™

One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells

Catalog number: 12297016
Catalog Number
12297016
Unit Size
21 x 50 µL/tube
Price (USD)
200.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
1229701621 x 50 µL/tube
200.00
Your Price:
***
Product Overview
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One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain. DH5α-T1R carry the tonA genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyse E. coli hosts, which are commonly used for cloning and library construction. They offer added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic. In addition to phage resistance, DH5α-T1R E. coli offer the following benefits:

• Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
• Reduced occurrence of unwanted recombination in cloned DNA (recA1)
• Efficient transformation of unmethylated DNA from PCR applications (hsdR)
• Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15)

Convenient and efficient format
DH5α-T1R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain
DH5α
Blue/White Screening
Yes
Cloning Methylated DNA
No
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Competent Cell Type
Chemically Competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
T1 Phage - Resistant (tonA)
Yes
Transformation Efficiency
> 1x10^9
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Format
One Shot
Product Line
One Shot™
Product Type
Competent Cells
Quantity
20 x 50 µL
Species
E. coli
Improves Plasmid Quality
Yes

Contents & Storage

Contains:
• One Shot MAX Efficiency DH5α-T1R Competent Cells: 20 vials, 50 µl each ( total of 1 ml)
• pUC19 DNA (10 pg/ul): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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Citations & References