TaqMan™ Rodent MicroRNA A Array v2.0
TaqMan™ Rodent MicroRNA A Array v2.0
Applied Biosystems™

TaqMan™ Rodent MicroRNA A Array v2.0

The Applied Biosystems™ TaqMan™ Rodent MicroRNA A Array v2.0 contains 384 TaqMan™ MicroRNA Assays enabling accurate quantitation of 335 andRead more
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Catalog NumberQuantity
43989674 pack
Catalog number 4398967
Price (USD)
3,165.00
Each
Add to cart
Quantity:
4 pack
Price (USD)
3,165.00
Each
Add to cart
The Applied Biosystems™ TaqMan™ Rodent MicroRNA A Array v2.0 contains 384 TaqMan™ MicroRNA Assays enabling accurate quantitation of 335 and 226 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan™ MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan™ MicroRNA Assay not related to human is included as a negative control. Details of the included assays is provided in the Array Map file. Purchase of the Megaplex™ RT Primers, Rodent Pool A is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool A for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan™ MicroRNA Arrays
provide all the advantages of TaqMan™ MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan™ MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the the TaqMan™ MicroRNA Array v2.0. When sensitivity is of the utmost importance, and/or sample is limiting Megaplex™ PreAmp Primers provide an optional preamplification step. TaqMan™ MicroRNA Arrays v2.0 and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v10 to be created in as little as 5 hours, providing the ideal miRNA profiling solution.

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex RT Primers and optional preamplification using Megaplex PreAmp Primers, TaqMan™ Universal PCR Master Mix is simply combined with each reaction and pipetted into each of the eight sample loading ports in the TaqMan™ Array, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase v10
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan™ MicroRNA Arrays v2.0. The content is derived from miRBase v10, and provides close to full coverage of Sanger with the most up-to-date TaqMan™ MicroRNA Assays. Each array (either human or rodent) contains a selection of carefully chosen endogenous control assays, providing you with a variety of options for data normalization. Of particular note is the inclusion of a new mammalian U6 assay, repeated four times on each card, and an assay unrelated to mammalian species, ath-miR159a, providing a process control.

Ideal for miRNA Profiling
The highly streamlined workflow—coupled with the high specificity, sensitivity, and dynamic range of TaqMan™ Assays—make this product solution ideal for miRNA profiling, especially when compared to microarrays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)AB 7900 HT 384-Well System
FormDried Down
Includes384-Well Arrays, Information CD
Internal Probe ModificationMGB (Minor Groove Binder) (3in), Non-Fluorescent Quencher (3in), FAM (5in)
Label or DyeFAM
Product LineTaqMan™
Quantity4 pack
Reaction SpeedStandard
Research CategoryGene Regulation, Epigenetics
TypeRodent MicroRNA A Array
Format384-well Microfluidic Card
Number of Arrays4 arrays
SpeciesMouse, Rat
Unit SizeEach
Contents & Storage
Contents: 384-well arrays and information CD.Storage: The TaqMan™ MicroRNA Arrays are shipped at ambient temperature. Upon arrival, store arrays at 2 to 8 ° and keep them protected from light.

Frequently asked questions (FAQs)

Why does the negative control well show amplification when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents or the cDNA template are contaminated. Please follow established PCR laboratory best practices.

Why do I have poor reproducibility across technical replicates when doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards?

Most likely the reagents were not adequately mixed. Ensure that all samples and reagents are mixed well.

When doing microRNA analysis using Megaplex Primer Pools and TaqMan Array Cards, why are the Ct values for the endogenous controls highly variable across the sample set?

Highly variable values for endogenous controls is most likely due to biological variation. We recommend that you use an alternative endogenous control, such as a non-variable miRNA.

Why is the Ct value for the no-template control (NTC) <35 for some TaqMan microRNA Assays?

The Ct value for the NTC can be <35 for the following reasons:
- There are non-specific interactions between primers. For NTC information on a specific assay, see the Megaplex Assay Performance File.
- The cDNA template is contaminated. Please ollow established PCR good laboratory practices to prevent contamination.
- The preamplification product was not diluted properly before real-time PCR. Ensure that you dilute the preamplification product according the procedure in the user guide.

Why can I not detect any miRNA in my sample using the TaqMan MicroRNA Assay?

Most likely, the threshold is set too high to detect miRNA in samples with low levels of expression. We recommend adjusting the threshold (Ct) or NOAMP flag threshold (Crt) to an appropriate level.