TaqMan® Rodent MicroRNA A Array v2.0

Catalog number: 4398967

Applied Biosystems™  Related applications: Real Time PCR (qPCR) | miRNA & Non-Coding RNA Analysis

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Description

The Applied Biosystems® TaqMan® Rodent MicroRNA A Array v2.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 335 and 226 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan® MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details of the included assays is provided in the Array Map file. Purchase of the Megaplex™ RT Primers, Rodent Pool A is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool A for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan® MicroRNA Arrays
provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the the TaqMan® MicroRNA Array v2.0. When sensitivity is of the utmost importance, and/or sample is limiting Megaplex™ PreAmp Primers provide an optional preamplification step. TaqMan® MicroRNA Arrays v2.0 and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v10 to be created in as little as 5 hours, providing the ideal miRNA profiling solution.

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex RT Primers and optional preamplification using Megaplex PreAmp Primers, TaqMan® Universal PCR Master Mix is simply combined with each reaction and pipetted into each of the eight sample loading ports in the TaqMan® Array, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase v10
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® MicroRNA Arrays v2.0. The content is derived from miRBase v10, and provides close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays. Each array (either human or rodent) contains a selection of carefully chosen endogenous control assays, providing you with a variety of options for data normalization. Of particular note is the inclusion of a new mammalian U6 assay, repeated four times on each card, and an assay unrelated to mammalian species, ath-miR159a, providing a process control.

Ideal for miRNA Profiling
The highly streamlined workflow—coupled with the high specificity, sensitivity, and dynamic range of TaqMan® Assays—make this product solution ideal for miRNA profiling, especially when compared to microarrays.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Dried Down
Format: 384-well microfluidic card
Species: Mouse, Rat
Label or Dye: FAM™
Product Size: 4 pack
Reaction Speed: Standard
Research Category: Epigenetics, Gene Regulation
Internal Probe Modification: MGB (Minor Groove Binder) (3'), FAM (5'), Non-Fluorescent Quencher (3')

Contents & storage

Contents: 384-well arrays and information CD. Storage: The TaqMan® MicroRNA Arrays are shipped at ambient temperature. Upon arrival, store arrays at 2 to 8 ° and keep them protected from light.

Documents

Manuals & protocols