DyLight™ 554-R0 NHS Ester

Catalog number: 46612

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

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Description

DyLight 554-R0 provides bright fluorescent detection for imaging. This rhodamine-based dye has excitation and emission peaks at 553 and 581 nm, respectively (in ethanol), and an extinction coefficient of 100,000 M-1cm-1. The amine-reactive DyLight 554-R0 NHS Ester allows for simple modification of antibodies, proteins, peptides, and other biomolecules through amide bond formation.

General features of DyLight yellow-emitting specialty dyes:

NHS ester reactive group—allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
Multiple solubility options—choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application

Dyes can be selected based upon their characteristic excitation and emission properties or relative hydrophilicity and hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential.

Criteria to consider when choosing a DyLight Yellow-emitting Specialty Dye
• Excitation and emission wavelengths—choose the best dye to match the excitation and emission capabilities of your instrument
• Water solubility—choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.

Applications:
• Fluorescence imaging
• Fluorescence correlation spectroscopy
• Flow cytometry
• Imaging
• Antibody labeling
• Direct immunofluorescence staining
• ELISA
• Western blotting
• Protein microarrays
• Polymer labeling
• Peptide labeling
• Phalloidin labeling for actin staining

Related Products
DyLight™ 554-R1 NHS Ester
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: DyLight™ Fluor (Specialty)
Chemical Reactivity: Amine
Solubility: DMF (Dimethylformamide)
Product Size: 1 mg
Excitation⁄Emission (nm): 553⁄581

Contents & storage

Store desiccated at -20°C. Shipped at ambient temperature.

Documents

Manuals & protocols