The PCM-3000 can be used for monitoring gradient formation in salt- or pH-gradient-based ion-exchange chromatography (IEC), typically used for biopharmaceutical analysis.
pH gradients are often used for proteins and large molecules where polarity differences alone can’t resolve different species with a typical organic gradient.
IEC is a versatile separation technique for profiling the charge heterogeneity of biotherapeutic proteins, including monoclonal antibodies (MAbs). The elution can be driven by pH, ionic strength, or a combination of these two parameters. The PCM-3000 can also be used to find the optimal elution conditions for specific proteins and MAbs.