The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from gDNA input. The assay can be used for basic and translational research to identify T-cell clones since the nucleotide sequence of the CDR3 region is unique to each T cell and codes for the part of the TCR beta chain that is involved in antigen recognition. For RNA samples, please see Cat. No. A45489.
The high sensitivity and specificity of the assay enables key applications in immunology, immuno-oncology, and vaccine research. The high-sensitivity dual-barcode indexing, flexible input requirements (100 ng–1 µg), high-depth sequencing, and high-throughput capability make this assay ideal for testing a variety of use cases in mouse models, including the role of T cells in generating immune response to immunotherapies such as checkpoint blockade inhibitors, cancer vaccines, or chimeric antigen receptor (CAR) T-cell therapies.
Benefits of the Ion AmpliSeq Mouse TCR Beta SR Assay, DNA, include: • Compatibility with a vast array of research sample types, including fresh-frozen and FFPE tissue, whole blood, peripheral blood leukocytes, and peripheral blood mononuclear cells • High sensitivity and low limit of detection for rare clone identification through dual-barcode indexing • Efficient workflow with 48-hour sample-to-results time • Flexible input requirements ranging from 100 ng to 1 µg • Streamlined and user-friendly informatics solution with multi-sample analysis functionality
The Ion AmpliSeq TCR Beta-SR Mouse Kit, DNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow from library preparation to analysis of samples can be accomplished in two days using the Ion Chef templating system and the Ion GeneStudio S5 sequencing system.