Our Oregon Green™ 488 conjugate of NeutrAvidin™ biotin-binding protein - a form of avidin that has been processed to remove carbohydrate and to lower its isoelectric point - can substantially decrease background due to nonspecific binding. The method used to deglycosylate the avidin retains its specific binding. This bright, green-fluorescent reagent (absorption/emission maxima ∼496/524 nm) is ideal for detecting biotinylated targets in fluorescence microscopy and flow cytometry applications.