Invitrogen™

MultiShot™ TOP10 Chemically Competent E. coli

Catalog number:  C40005

Related applications:

Transformation

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Description

MultiShot™ TOP10 Chemically Competent E. coli provide transformation efficiencies of >1 x 108 cfu/µg control plasmid DNA, and are suited for high-throughput transformations. MultiShot™ TOP10 Chemically Competent cells are packaged in five 96-well microtiter plates (15 µL aliquots) to simplify high-throughput bacterial transformations. Benefits of MultiShot™ TOP10 cells:

• Designed for high-throughput applications—microtiter plate format designed for automated high-throughput cloning
• Compatible with genomic DNA—the mcrA mutation supports efficient transfection of methylated DNA
• Permits rapid screening—the LacZΔM15 allele permits rapid blue/white screening of transfectants on plates containing X-Gal or Bluo-Gal
• Efficient—the endA1 mutation helps reduce endonuclease activity, improving subsequent plasmid DNA yields
• Reliable—the recA1 mutation helps reduce unwanted recombinationEasy to use for high-throughput transformations
MultiShot™ TOP10 Chemically Competent cells are genetically similar to the reliable DH10B™ strain, and are available in five 96-well plates to facilitate high-throughput transformation. After addition of DNA, MultiShot™ TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler. Using our TA Cloning® and TOPO® Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Find the strain and format that you need
We also offer many other different strains and formats of chemically competent cells and electrocompetent cells to help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection of MultiShot™ formatted comp cells or contact us for custom formatting options.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: TOP10
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F' Episome (to make ssDNA): Lacks F' Episome
Format: MultiShot
High Throughput Compatibility: High Throughput-Compatible
Improves Plasmid Quality: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: MultiShot™
Product Size: 5 plates
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Species: E. coli
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^8

Contents & storage

Contains:
• MultiShot™ TOP10 Chemically Competent E. coli: Five 96-well plates, 15 µl per well
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 5 bottles, 15 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.