ProcartaPlex™ Human Immune Monitoring Panel, 65plex
ProcartaPlex™ Human Immune Monitoring Panel, 65plex
Citations et références (13)
Invitrogen™

ProcartaPlex™ Human Immune Monitoring Panel, 65plex

Le panneau Human Immune Monitoring 65-Plex ProcartaPlex permet l’étude de la fonction immunitaire en analysant 65 cibles protéiques dans unAfficher plus
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RéférenceQuantité
EPX650-10065-90196 dosages
Référence EPX650-10065-901
Prix (EUR)
11 900,00
Each
Ajouter au panier
Quantité:
96 dosages
Prix (EUR)
11 900,00
Each
Ajouter au panier
Le panneau Human Immune Monitoring 65-Plex ProcartaPlex permet l’étude de la fonction immunitaire en analysant 65 cibles protéiques dans un seul puits à l’aide de la technologie Luminex xMAP. Analysez simultanément 65 cibles de cytokine, de chimiokine et de facteur de croissance pour un profilage efficace de la réponse immunitaire, de la découverte de biomarqueurs et de la validation. Le panneau est fourni dans un format prêt à l’emploi avec des flacons individuels de réactifs de capture et de détection formulés à une concentration de 1X, nécessitant moins de pipetage et de configuration expérimentale. Ce kit n’est pas compatible avec l’instrument MAGPIX. Les utilisateurs de MAGPIX doivent utiliser le panneau plex de surveillance de l’immuno-surveillance humaine 65 plex pour MAGPIX (Réf N° EPX650-16500-901) comprenant deux kits distincts : un kit 43-plex et un kit 22-plex spécifiquement conçus pour être utilisés sur l’instrument MAGPIX capable de détecter jusqu’à 50 cibles simultanément.

Les panneaux préconfigurés ProcartaPlex sont largement testés pour la combinabilité, l’interférence et la réactivité croisée des analyses afin de fournir le plus haut niveau de validation et de précision. Tous les panneaux ProcartaPlex sont fournis avec les réactifs nécessaires pour effectuer le dosage.

Les panneaux multiplex ProcartaPlex sont disponibles en plusieurs formats et peuvent être utilisés sur six espèces (humain, souris, rat, primate non humain, porcin et canin).

Cible [région de microbille] :
Cytokines : G-CSF (CSF-3) [42], GM-CSF [44], IFN alpha [48], IFN gamma [43], IL-1 alpha [62], IL-1 bêta [18], IL-2 [19], IL-3 [73], IL-4 [20], IL-5 [21], IL-6 [25], IL-7 [26], IL-8 (CXCL8) [27], IL-9 [52], IL-10 [28], IL-12p70 [34], IL-13 [35], IL-15 [65], IL-16 [70], IL-17A (CTLA-8) [36], IL-18 [66], IL-20 [81], IL-21 [72], IL-22 [76], IL-23 [63], IL-27 [14], IL-31 [37], LIF [15], M-CSF [67], MIF [53], TNF alpha [45], TNF bêta [54], TSLP [80]

Chimiokines : BLC (CXCL13) [29], ENA-78 (CXCL5) [82], Éotaxine (CCL11) [33], Éotaxine-2 (CCL24) [37], Éotaxine-3 (CCL26) [77], Fractalkine (CX3CL1) [59], Gro-alpha (CXCL1) [61], IP-10 (CXCL10) [22], I-TAC (CXCL11) [57], MCP-1 (CCL2) [51], MCP-2 (CCL8) [8], MCP-3 (CCL7) [68], MDC (CCL22) [87], MIG (CXCL9) [69], MIP-1 alpha (CCL3) [12], MIP-1 bêta (CCL4) [47], MIP-3 alpha (CCL20) [56], SDF-1 alpha (CXCL12) [13]

Facteur de croissance / régulateurs : FGF-2 [75], HGF [46], MMP-1 [64], NGF bêta [55], SCF [39], VEGF-A [78]

Récepteurs solubles : APRIL [88], BAFF [86], CD30 [84], CD40L (CD154) [74], IL-2R (CD25) [22], TNF-RII [85], TRAIL (CD253) [58], TWEAK [97]

À propos des dosages ProcartaPlex pour la plateforme Luminex
Les immunoessais ProcartaPlex sont basés sur les principes d’un ELISA de type sandwich, avec l’utilisation de deux anticorps hautement spécifiques se liant à différents épitopes d’une protéine pour quantifier simultanément toutes les cibles protéiques à l’aide d’un instrument Luminex. Les dosages multiplex ProcartaPlex nécessitent seulement seulement 25 µl de plasma ou de sérum, ou 50  µl de surnageant de culture cellulaire, et seulement quatre heures pour obtenir les résultats analysés.

Caractéristiques :
• Résultats reproductibles et fiables — validés en panneau selon la norme la plus élevée de l’industrie, avec des tests de combinabilité des cibles protéiques et de réactivité croisée
• Plus de résultats par échantillonneur — permet de réaliser simultanément plusieurs cibles protéiques dans un seul échantillon de 25 – 50 µl
• Plate-forme de multiplexage à haute référence avec la technologie bien établie Luminex, pour la détection et la quantification des protéines

Les dosages ProcartaPlex utilisent Luminex xMAP (profilage multianalyte) Technologie pour la détection et la quantification simultanées de jusqu’à 80 cibles protéiques dans un seul échantillon de 25–50 µl — de plasma, de sérum, de surnageant de culture cellulaire et d’autres liquides corporels.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Plage de dosageVoir le certificat d’analyse
Sensibilité du dosageVoir le certificat d’analyse
Méthode de détectionFluorescent
À utiliser avec (équipement)Instruments Luminex™
FormatKit multiplex
Gamme de produitsProcartaPlex
Type d’échantillonSérum, plasma, surnageants de culture cellulaire (SCC), Plasma, Cell Culture Supernatants
Volume d’échantillonSérum, plasma : 25 μl ; CCS : 50 μl
Conditions d’expéditionGlace humide
CombinabilityNon combinable
Type de produitPanneau multiplex
Quantité96 dosages
Research AreaImmunology
EspècesHumaine
Unit SizeEach
Contenu et stockage
  • 2 flacons de mélange étalon humain M (lyophilisée)
  • 1 flacon de mélange de microbilles de capture (1X)
  • 1 flacon de mélange d’anticorps biotinylé (1X)
  • 2 flacons de contrôle humain-Élevé
  • 2 flacons de contrôle humain-Bas
  • 1 flacon de tampon de lecture (1X)
  • 1 flacon de tampon de lavage (10X)
  • 1 flacon de streptavidine-PE (1X)
  • 1 flacon de tampon de dosage universel (1X)
  • Barrettes de 8 tubes
  • Film adhésif
  • Plaque 96 puits à fond plat, noire
  • Stocker de 2°C à 8°C

Foire aux questions (FAQ)

What is the size of the Luminex beads you currently use?

The beads used in our Luminex instrument-compatible ProcartaPlex and QuantiGene Plex assays are 6.5 micron superparamagnetic beads.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am interested in performing Luminex assays using BioSource kits, and I have a Luminex xMAP system. Besides the kits and system, what other reagents and equipment will I need?

The following is a list of general lab supplies that are required for running BioSource immunoassays on the Luminex xMAP system:
1) Sonicating water bath
2) Orbital shaker
3) Vortexer
4) Repeating and/or multi-channel pipetter (not required, but recommended)
5) Calibrated adjustable precision pipettes, with disposable plastic tips
6) Glass/plastic tubes and racks for preparing reagents
7) Graduated cylinder and container for preparing wash solution
8) Aluminum foil
9) Deionized or distilled water.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the Luminex beads require special care in handling?

The Luminex beads should be protected from light because they are susceptible to photobleaching. We recommend protecting the beads by keeping containers covered with aluminum foil during all incubation steps, and exercising care during handling. The beads should not be frozen, subjected to excessive heat, or exposed to organic solvents.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why would the Luminex acquisition software display "Sample Empty" messages during analysis?

(1) The user did not properly aliquot the diluted beads, such that no beads were actually added to the wells (make sure that the bead concentrates are sonicated and vortexed well, then check the pipet tip to ensure that air bubbles were not drawn up)
(2) The user missed loading diluted beads to some wells, which is likely since the small volume is clear and difficult to visualize in the clear plastic plate (we have now addressed this customer difficulty by coloring each of the Buffer Reagent Kit components)
(3) The user applied too much vacuum pressure at some point during the wash steps, or allowed the pressure to spike even once, such that the filter membrane tore in a few wells releasing the beads (make sure that the vacuum manifold pressure is kept below 5mm/in Hg, depending on their system -- a good rule of thumb is that it should take a full 3-second count to GENTLY empty the wells of 200uL)
(4) The user did not properly sonicate and vortex the beads prior to dilution, such that the percent of bead aggregation was high and the instrument was unable to find enough single beads to meet the events/bead value designated by the customer (make sure that the Bead Concentrate tube is put into the waterbath all the way to the cap, since the tube is hollow until the top third)
(5) The user lost beads by shaking the plate too aggressively or handling it improperly (make sure that the orbital shaker is set to a speed that allows for maximum vortex in the wells without spillage)
(6) The user exposed the beads to an excess of light during storage or running of the assay, such that some but not all of the beads were photobleached and therefore falling outside the acceptable range for each bead region (make sure that the plate is covered on the top/sides with foil throughout the assay, away from Windows and spotlights, and that the bead component of the kits is stored in the dark)*
(7) There was a clog in the sample needle, such that the instrument was unable to take up enough sample to meet the number of events requested per bead region (suggest that the user follow the manual instructions for dislodging a clog, which include several Back Flush steps and may require removal of the needle for sonication with probe alignment).

* Some of the older Antibody Bead Kits still have clear plastic tops instead of black ones. In cases where customers store kits in lit refrigerators, or keep them open on the lab bench, even a few hours of light exposure is enough to photobleach beads. It is important to note, in general, that higher number bead regions are more susceptible to photobleaching. In order to draw conclusions about the source of the difficulty, we would ask to see the data, specifically the Masterplex QT file, which would enable us to examine the pattern of "Sample Empty" occurrences in addition to the bead counts per well.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the Luminex beads made of?

The beads are made of polystyrene.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all ProcartaPlex™ Human Immune Monitoring Panel, 65plex FAQs

Citations et références (13)

Citations et références
Abstract
No Distinct Cytokine, Chemokine, and Growth Factor Blood Profile Associated With Monkeypox Virus Clade IIb Infected Patients.
Authors:Bangwen E,Berens-Riha N,de Vrij N,Ceulemans A,Brosius I,De Vos E,Pham TT,Bottieau E,van Griensven J,Netongo PM,Van Esbroeck M,Vercauteren K,Van Dijck C,Adriaensen W,Liesenborghs L
Journal:Journal of medical virology
PubMed ID:40156483
Previous studies indicated Clade I monkeypox virus infection to be associated with marked elevation of proinflammatory cytokines. This remains unexplored for Clade II‐associated disease, which has different clinical manifestations and prognosis. We used a 65‐plex cytokine, chemokine, and growth factor (CCG) panel to analyze serum samples of 100 male acute ... More
Immune monitoring of trabectedin therapy in refractory soft tissue sarcoma patients - the IMMUNYON study.
Authors:Rodrigues-Santos P,Almeida JS,Sousa LM,Couceiro P,Martinho A,Rodrigues J,Fonseca R,Santos-Rosa M,Freitas-Tavares P,Casanova JM
Journal:Frontiers in immunology
PubMed ID:40007535
Soft tissue sarcomas (STS) encompass over 50 histologic subtypes, representing more than 1% of solid tumors. Standard treatments include surgical resection and therapies such as anthracyclines or trabectedin for advanced cases, though challenges persist due to the tumor microenvironment’s complexity and limited immune profiling data. This study evaluates Trabectedin therapy ... More
Immune profiling of human vestibular schwannoma secretions identifies TNF-α and TWEAK as cytokines with synergistic potential to impair hearing
Authors:Vasilijic S, Seist R, Yin Z, Xu L, Stankovic KM
Journal:Journal of Neuroinflammation
PubMed ID:39923035
Vestibular schwannoma (VS) is an intracranial tumor arising from the Schwann cells of the vestibular nerve and is an important cause of sensorineural hearing loss (SNHL) in humans. The mechanisms underlying this SNHL are incompletely understood and currently, there are no drugs FDA approved specifically for VS. This knowledge gap ... More
Assessing serum cytokine profiles in inflammatory breast cancer patients using Luminex® technology.
Authors:Yaacoubi-Loueslati B
Journal:Cytokine
PubMed ID:37918053
Inflammatory breast cancer (IBC), accounts for the majority of deaths associated with breast tumors. Because this form is aggressive from its appearance and has a strong metastatic potential. The majority of patients are not diagnosed until late stages, highlighting the need for the development of novel diagnostic biomarkers. Immune mediators ... More
Multiplexing protein and gene level measurements on a single Luminex platform.
Authors:Cook DB, McLucas BC, Montoya LA, Brotski CM, Das S, Miholits M, Sebata TH
Journal:Methods
PubMed ID:30742996
'The ability to simultaneously measure multiple secreted proteins and the corresponding gene expression levels from a single sample is valuable for comprehensive analysis. Bottlenecks to traditional immunoassays and gene expression assays include large sample consumption, time consuming experimental procedures, and complex data analysis.' ... More
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