Gibco Human Spheroid-Qualified Hepatocytes are off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of five million viable cells, sufficient for up to sixteen 96-well plates of hepatic spheroids.
• Maintain cells in culture longer—up to 21 days, >4X longer than cells last in monolayer culture • Design your own schedule—cells are ready for plating when you are • Interrogate pathways of interest—applicable for toxicity, metabolism, or disease modeling
Simplified protocol Cryopreserved cells can be thawed and plated at your convenience, in as little as 60 minutes. Try it on your own, or request a demo with an experienced scientist.
Stringent quality control Our high standards help ensure only the best spheroid-forming lots become available, maximizing your chance of success. In addition, each lot is tested for: • Albumin production in monolayer and spheroid culture • Phase I enzyme induction • Bile duct formation (CFDA staining) • Proper morphology • Viability >75% • Attachment efficiency • Monolayer formation and integrity
Important! For best results, do not attempt hepatic spheroid formation in low attachment plates from other sources. Hepatocyte lots are qualified in Nunclon Sphera plates only and use of other plates can lead to variability in spheroid formation.
Flexible readouts Analyze single spheroids or pool several for detection in assays such as gene expression, immunostaining, viability, or CYP activity.
For Research Use Only. Not for use in diagnostic procedures.