Thermo Scientific™

UltiMate™ 3000 Semi-Preparative System

Related applications:

Industrial Chromatography

Purify compounds from microgram to lower-milligram quantities with accurate gradients and flexible flow rates. The Thermo Scientific™ UltiMate™ 3000 Binary Semi-preparative system is tailored for robust, safe, and high-performance purifications. Based on proven high-pressure gradient principles, it takes full advantage of Dionex UltiMate 3000 features such as SmartFlow™ pulse-free eluent delivery and wide linear range detection. The semi-preparative autosampler delivers milliliter injection volumes while minimizing backpressure contributions to support high flow rates at low backpressures.
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Recommended Use

Typical applications include the purification of side products and impurities for structure elucidation, purification of compounds from natural product extracts, and purification of compound libraries for pharmaceutical discovery. It is recommended for semi-preparative separations on 4.6–20mm I.D. columns.


  • Wide operating flow rate range from 0.5 to 50mL/min
  • SmartFlow technology for precise and accurate flow rates
  • High-pressure proportioning at high accuracy and precision
  • Variable mixing volumes for different flow and delay volume requirements
  • Sample injections up to 2.5mL with excellent volumetric linearity
  • For isocratic applications, Double Flow mode enables flow rates of up to 100mL/min.
  • Solvent and waste level monitoring with Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System
  • Compatible with Chromeleon 7.x eWorkflow walk-up solution and advanced reporting features
  • Automated peak fractionation and compound mass based collection with Chromeleon soiftware

Choose the Right Configuration for You Semi-preparative binary pump (HPG-3200BX)

  • Solvent rack for large solvent reservoirs (SR-3000)
  • Thermostatted or nonthermostatted semi-preparative autosampler (WPS-3000TSL, WPS-3000SL)
  • Diode array, multiple wavelength, variable wavelength, fluorescence, charged aerosol, refractive index or mass spectrometry detectors with semi-prep flow cells or after flow splitting
  • Viper fingertight fitting technology connections for system plumbing (up to 180µm ID only)
  • pH and conductivity monitoring (PCM-3000)
  • Fraction collector (AFC-3000)
  • Chromeleon 7.2 software packages (standalone, networked, remote data processing and/or control)