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Guanidine hydrochloride, Molecular Biology Grade
Catalog number: J65661.22
100 g, Each
Thermo Scientific Chemicals
Guanidine hydrochloride, Molecular Biology Grade
Catalog number: J65661.22
100 g, Each
Quantity
Catalog number: J65661.22
also known as J65661-22
Price (USD)
Price: 89.30
Online price: 75.65
Your price:
Quantity
-
Chemical Identifiers
CAS
50-01-1
IUPAC Name
hydrogen guanidine chloride
Molecular Formula
CH6ClN3
InChI Key
PJJJBBJSCAKJQF-UHFFFAOYSA-N
SMILES
[H+].[Cl-].NC(N)=N
Specifications
Specification SheetAssay (unspecified)
Absorbance (260nm, 6M in water): 0.03 max.
Form
Powder
Assay (Titration)
99.5% min.
DNase
None detected
Impurity content
Arsenic: 5ppm max.
Description
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
RUO – Research Use Only
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