Neon® Transfection System

Catalog number: MPK5000

Invitrogen™  Related applications: RNAi | Transfection

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Description

The Neon® Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 × 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon® Transfection System is:

Efficient—up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells
Flexible—easily transfect from 2 × 104 cells to 6 × 106 cells per reaction
Simple—easy to use, with a single reagent kit for all cell types
Versatile—open system allows electroporation parameters to be optimized freely

One-of-a-kind electroporation
Unlike standard cuvette-based electroporation chambers, the Neon® system uses a patented, biologically compatible pipette tip chamber that generates a more uniform electric field. Neon® Tips are components of the Neon® Transfection System Kits, available in 100 µL and 10 µL formats for large- and small-scale transfections (see a list of Neon® Transfection System Kits and Accessories). This design allows better maintenance of physiological conditions, resulting in very high cell survival compared to conventional electroporation (see figure).

Plug-n-play out of the box
The transfection process couldn’t be simpler. Draw up the cells and plasmid mix into the Neon® pipette tip, plug into the pipette holder, and press start. Then just pipette the transfected cells into your culture vessel. No more filling and pulling sample from the cuvette and capping and de-capping. The Neon® system uses a simple 3-step transfection procedure. The transfection occurs in the Neon® pipette tip.

Perform more transfections with limited or precious cells
Your cells may be limited when you are required to perform neuronal preps or when enriching for homogeneous cell populations via FACS or Dynabeads® enrichment. In these instances, reducing the number of cells required per transfection can allow you to perform more complex experiments with your precious cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Electroporation Pipette Tip
System: Neon® Transfection System
Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells
Power Limit: 300 W
Product Size: 1 each
Current Limit: 3.0-6.0 A
Input Voltage: 100-240 VAC
Reaction Time: 1 sec
Voltage Limit: 0.5-2.5 kV
Classification: Animal Origin-Free, Reduced Serum, Chemically Defined, Low-Protein, Reduced Growth Factors, Xeno-Free
Serum Compatible: Yes
Shipping Condition: Room Temperature
Compatible Products: Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors
Sample Loading Volume: Depends on kit used
Sample Type (Specific): Plasmid DNA, Synthetic siRNA
Purity or Quality Grade: Transfection Grade
For Use With (Equipment): Neon® Transfection System
Starting Material (Cell #): 2x10^4 - 6 x10^8 cells; Varies by cell type

Contents & storage

The Neon® Transfection System comes with a Neon® electroporation device, pipette, pipette station, USB thumbdrive, manual and power cord.

For use at room temperature under normal laboratory conditions.

Documents

Manuals & protocols

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