Invitrogen™

SYTO™ Deep Red Nucleic Acid Stain, for live cells

Catalog number:  S34901

 Related applications:

Cell Viability, Proliferation & Function

| Flow Cytometry

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Description

SYTO Deep Red Nucleic Acid Stain is cell-permeant dye that specifically stains the nuclei of live, dead, or fixed cells. It can be used in live-cell fluorescent imaging workflows and in fixed cell workflows, including immunocytochemistry, immunohistochemistry, and immunofluorescence experiments. After a brief incubation with the stain, the nuclei of live, dead, or fixed cells fluoresce with a deep red/far red signal that is detectable with a Cy5/deep red standard filter set or laser configuration.

• Cell-permeant dye that stains nuclei of live, dead, or fixed cells
• Detectable with deep red/Cy5 fluorecence filter set, with excitation/emission maxima of 652/669 nm
• Five times greater fluorescent signal when staining dsDNA vs RNA

SYTO Deep Red Nucleic Acid Stain can be mulitplexed with blue, green, orange, red, and near-IR fluorophores when compatible fluorescent filter/laser configurations are used. The stain increases in fluorescence with increasing concentrations of dsDNA. SYTO Deep Red Nucleic Acid Stain displays up to five times more fluorescence when incubated with dsDNA vs RNA, unlike DRAQ or other live-cell deep red nucleic acid stains. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make the stain ideal as a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence microscopes, fluorimeters, fluorescence microplate readers, and flow cytometers. The stain has been successfully used on live monolayer cells and spheroid cells and as a nuclear counter-stain in immunocytochemistry.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sub-Cellular Localization: Nucleus
Excitation⁄Emission (nm): 652⁄669
Product Line: SYTO™
Product Size: 5 vials
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

• 5 vials SYTO Deep Red Nucleic Acid Stain (makes 2 mM solution at 2000X concentration when dissolved in 50 µL DMSO/vial)
• 5 vials Probenecid (77 mg/vial; lyophilized)

When stored at ≤–20°C in the dark, the product is stable for 6 months after reciept.

Documents

Manuals & protocols