Total protein normalization is a useful method for obtaining accurate, quantitative western blotting data, as housekeeping proteins can often be affected by experimental conditions. The Invitrogen No-Stain Protein Labeling Reagent is a fast, easy to use, covalent protein labeling reagent, applied to...
Learn More Metabolic Labeling Reagents for Proteins
Metabolic labeling refers to methods in which the endogenous synthesis and modification machinery of living cells is used to incorporate detection or affinity tags into biomolecules. Typically, this is accomplished by culturing cells or organisms in media in which a specific natural molecular...
Learn More Protein Gel Electrophoresis
Protein gel electrophoresis is a simple way to separate proteins prior to downstream detection or analysis. Our portfolio of high-quality protein electrophoresis products unites gels, gel tanks, protein gel handcast system, stains, molecular weight markers and standards, running buffers, and...
Learn More Overview of Protein Labeling
Protein labels Biomolecule labeling strategies Recommended reading, Biotinylation Overview of Protein Labeling Enzyme-Protein Conjugation Fluorescent Protein Labeling, Biotin is a useful label for protein detection, purification and immobilization because of its extraordinarily strong binding to...
Learn More Fluorescent Protein Labeling
The attachment of fluorescent dyes to proteins and other biomolecules is a common strategy used to better understand protein structure and function. These fluorescent proteins can used in a broad range of applications including immunochemistry, fluorescence in situ hybridization (FISH), cell...
Learn More Protein Labeling and Crosslinking
We offer a wide range of products for the crosslinking, biotinylation, pegylation and fluorescent labeling of proteins, antibodies and peptides. These bioconjugation reagents are commonly used to studying the structure and interaction of proteins, build immunoassays, increase protein solubility or...
View all What is protein crosslinking? What is protein labeling? What functional groups are available for crosslinking/labeling? How can I store my protein after crosslinking/labeling?, View all After crosslinking/labeling, my protein of interest precipitates out of solution. What can I do?
Learn More Western Blot Videos
Western blotting is a multi-step process that can take hours or 1 to 2 days to complete, depending on the methods used. Below are videos that detail the common steps used to perform a western blot, from separating proteins in protein gel electrophoresis to developing the blot.
Learn More Foundation Library
Learn about methods and technologies for protein gel electrophoresis and western blot detection and imaging. Browse this foundation library content by topic to find overview articles, application notes, and technical tips.
Learn More DyLight Microscale Protein Labeling Kits
Thermo Scientific DyLight microscale protein labeling kits contain NHS ester-activated derivatives of our high-performance DyLight dyes designed specifically for labeling small quantities of antibodies or proteins.
Learn More Quantitative Western Blot Analysis
Traditional western blotting is useful for detecting the presence or absence of a specific protein in a lysate or complex mixture. Furthermore, it can provide a qualitative assessment of changes in protein expression levels in response to stimuli or interventions.
Learn More Fluorescent Protein Gel and Membrane Stains
Fluorescent stains are rapid, and highly sensitive for detecting total protein in protein electrophoresis gels and membranes. Fluorescent stains are designed for use in 1D and 2D PAGE and offer sensitivities similar to that obtained with silver staining techniques.
Optimize your protein labeling, crosslinking, and modification experiments to get the best results. We’ve compiled a detailed knowledge base of the top tips and tricks to meet your research needs. View the relevant questions below:, Browse our FAQ database for more information ›, Having problems...
We are dedicated to your success.Get back on track. View our expert recommendations for commonly encountered problem scenarios related to your protein labeling, crosslinking, and modification experiments.
Learn More Hear from Experts
Watch our learning series, which includes poster presentations and on-demand webinars (lectures), to receive firsthand knowledge of the latest technologies for protein gel electrophoresis and western blotting .