Semi-Preparative HPLC Systems

High-performance purifications

The Thermo Scientific UltiMate 3000 Binary Semi-preparative system is a workhorse that will always be ready to assist with your purification of natural product extracts, isolation of impurities, or other large-scale LC processes.

Browse our complete portfolio below, or let one of our experts help you tailor a selection specific to your application and workflow needs.

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UltiMate 3000 Semi-Preparative System

The UltiMate 3000 Binary Semi-preparative system is tailored for robust, safe, and high-performance purifications. Based on proven high-pressure gradient principles, the system takes full advantage of UltiMate 3000 features such as SmartFlow pulse-free eluent delivery and wide linear range detection.

This system is compatible with our full portfolio of preparative HPLC columns.


UltiMate 3000 Binary Semipreparative System configuration

System UltiMate 3000 Semi-Preparative System
Pump Biocompatible Binary Pump
HPG-3200BX
Autosampler WPS-3000TSL Semi-preparative
Column compartment TCC-3000SD
Automated fraction collector Fraction Collector FT/F

Compatible components for the UltiMate 3000 Semi-Preparative HPLC system

Multiple Detector Options Preparative HPLC Columns Chromeleon CDS Software
  • Charged aerosol detector
  • Diode array detector
  • Electrochemical detector
  • Fluorescence detector
  • Mass spectrometry detection
  • Multiple wavelength detector
  • Refractive index detector
  • Variable wavelength detector
  • Extensive portfolio of prep LC columns to enable easy scale up from analytical method
  • A wide variety of chemistries and particle sizes
  • Full range of column hardware with optional XtendedLife technology offers robust and reproducible performance
  • Streamlines your data processing
  • Industry-leading ease of use and intelligent functionality
  • Unique user guidance and hardware automation features designed for UltiMate 3000 systems

Discover the best HPLC systems for your applications and budget

Discover the best HPLC system for your applications and budget

Use these tools to help you decide

HPLC Systems Selection Guide
Use this tool to rank the top HPLC instruments for your applications based on your responses to simple pull-down menus.

HPLC System Total Cost of Ownership Calculator
Use this tool to estimate your cost for purchasing and running Thermo Scientific HPLC and UHPLC systems. You can compare costs between instruments.

LC Method Transfer Calculator
Use this online calculator to transfer methods from HPLC to UHPLC conditions. Calculate the new gradient table, adjust sample volumes and run times if applicable.


Thermo Fisher Cloud
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Learn more

AppsLab Library – The easy application note finder tool – Go to AppsLab >
Meet the UHPLC System that's built for biopharma – Learn more