Description: This MUNC33 monoclonal antibody reacts with mouse interleukin-25 receptor (IL-25R), also known as IL-17RB. IL-25R is a 55 kDa transmembrane protein that dimerizes with IL-17RA to form the functional receptor for IL-25 (IL-17E). IL-25R is detectable in various tissues, including the lung and gastrointestinal tract and exists as both membrane bound and soluble form. IL-25/IL-25R interaction has been shown to activate the NF-kB, MAPK, and JNK pathways as well as gene transcription by STAT6, GATA-3, and NFATc1. IL-25R signals in T helper 2 (Th2) cells, type 2 innate lymphoid (ILC2) cells and some iNKT cells to promote differentiation and effector function. Whereas IL-25-deficient mice have an impaired Th2 response to parasitic helminth infection, elevated expression of IL-25 and IL-25R is observed in patients with chronic asthma and atopic dermatitis.
Applications Reported: This MUNC33 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MUNC33 antibody has been tested by flow cytometirc analysis of mouse lymph node cells or IL-25R-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are IL-17B, IL-17C, and IL-17E. Like IL-17, IL-17B, C, and E are able to induce proinflammatory responses but don't bind to the IL-17 receptor suggesting additional IL-17R related receptors might exist. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8. IL-17Rh1 mRNA is highly expressed in kidney and liver and at moderate level in small intestine, colon, and brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ecotropic viral integration site 27; IL-17 receptor B; IL-17 receptor homolog 1; IL-17B receptor; IL-17ER; IL-17RB; interleukin 17B receptor; Interleukin-17 receptor B; Interleukin-17B receptor
Gene Aliases: Evi27; IL-17ER; IL-17Rh1; Il17br; Il17rb; IL17RH1
UniProt ID: (Mouse) Q9JIP3
Entrez Gene ID: (Mouse) 50905