Immunofluorescent analysis of 14-3-3 sigma showing staining in the cytoplasm of A431 cells. A431 cells were fixed in ice-cold Methanol for 5 min and stained using a 14-3-3 sigma polyclonal antibody (Product # PA5-27147) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 154 and 247 of Human 14-3-3 sigma|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27147 targets 14-3-3 sigma in IF, IHC (P), and WB applications and shows reactivity with Human, mouse and rat samples.
The PA5-27147 immunogen is synthetic peptide corresponding to a region within amino acids 154 and 247 of Human 14-3-3 sigma.
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. p53-regulated inhibitor of G2/M progression.
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