|Tested species reactivity||Tag|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant (His) 6-p53 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1 to 0.4 µg/ml|
|Flow Cytometry (Flow)||5-10 µl/10^6 cells|
|Immunocytochemistry (ICC)||1-4 µg/mL|
|Immunofluorescence (IF)||1-4 µg/mL|
|Immunohistochemistry (Frozen) (IHC (F))||1-4 µg/mL|
|Immunoprecipitation (IP)||4 ug/mL|
|Western Blot (WB)||1-4 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-4806 detects any kind of 6X-His tagged proteins in cells and complex cellular lysates.
MA1-4806 has been successfully used in flow cytometry, immunohistochemistry (frozen), immunofluorescence, Western blot, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. A higher number of histidine residues leads to an increased binding affinity of the antibody.
The MA1-4806 immunogen is recombinant (His) 6-p53 protein
Recombinant proteins are utilized for various purposes in molecular biology such as the production of antibodies for investigation of the mechanism of protein-nucleic acid or protein-protein interactions. Many prokaryotic expression vectors have been established enabling synthesis of the protein of interest as a fusion with a peptide, thus facilitating purification. Expression of recombinant proteins in E. coli as a fusion protein with neighboring histidine residues is one of the most popular methods, because the proteins have useful attributes. The affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.