Proteintech

ACSL1 Polyclonal Antibody

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ACSL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 13989-1-AP (ACSL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACSL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13989-1-AP ( ACSL1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ACSL1 Antibody in Western Blot (WB) — Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

ACSL1 Antibody in Immunoprecipitation (IP) — IP result of anti-ACSL1 (IP:13989-1-AP, 4 ug; Detection:13989-1-AP 1:300) with mouse liver tissue lysate 4000 ug.

Product Details

13989-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 13 publications 13 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ACSL1 Fusion Protein Ag5059 (349-698 aa encoded by BC050073) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.22 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages. It has 2 isoforms produced by alternative splicing.

Immunogen sequence: RLLMDDLKV LQPTVFPVVP RLLNRMFDRI FGQANTTLKR WLLDFASKRK EAELRSGIIR NNSLWDRLIF HKVQSSLGGR VRLMVTGAAP VSATVLTFLR AALGCQFYEG YGQTECTAGC CLTMPGDWTA GHVGAPMPCN LIKLVDVEEM NYMAAEGEGE VCVKGPNVFQ GYLKDPAKTA EALDKDGWLH TGDIGKWLPN GTLKIIDRKK HIFKLAQGEY IAPEKIENIY MRSEPVAQVF VHGESLQAFL IAIVVPDVET LCSWAQKRGF EGSFEELCRN KDVKKAILED MVRLGKDSGL KPFEQVKGIT LHPELFSIDN GLLTPTMKAK RPELRNYFRS QIDDLYSTIK V (349-698 aa encoded by BC050073)

Target Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ACS1; Acyl CoA synthetase, long chain; Acyl-CoA synthetase 1; Arachidonate--CoA ligase; fatty acid Coenzyme A ligase, long chain 2; fatty-acid-Coenzyme A ligase, long-chain 1; fatty-acid-Coenzyme A ligase, long-chain 2; L LACS2; LACS 1; LACS 2; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; long-chain fatty-acid-coenzyme A ligase 1; Long-chain-fatty-acid--CoA ligase 1; Long-chain-fatty-acid--CoA ligase, liver isozyme; OTTHUMP00000218457; OTTHUMP00000218460; OTTHUMP00000218462; OTTHUMP00000218465; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1; Phytanate--CoA ligase

Gene Aliases: Acas; Acas1; ACS; ACS1; ACSL1; Acsl2; COAA; FACL1; FACL2; FACS; LACS; LACS 1; LACS1; LACS2

UniProt ID: (Human) P33121, (Mouse) P41216, (Rat) P18163

Entrez Gene ID: (Human) 2180, (Mouse) 14081, (Rat) 25288

Molecular Function: ligase