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ACSL1 Polyclonal Antibody

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Antibody Testing Data (16)

ACSL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

ACSL1 Antibody (13989-1-AP) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 13989-1-AP (ACSL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACSL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ACSL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13989-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ACSL1 Fusion Protein Ag5059 (349-698 aa encoded by BC050073)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages. It has 2 isoforms produced by alternative splicing.

Immunogen sequence: RLLMDDLKV LQPTVFPVVP RLLNRMFDRI FGQANTTLKR WLLDFASKRK EAELRSGIIR NNSLWDRLIF HKVQSSLGGR VRLMVTGAAP VSATVLTFLR AALGCQFYEG YGQTECTAGC CLTMPGDWTA GHVGAPMPCN LIKLVDVEEM NYMAAEGEGE VCVKGPNVFQ GYLKDPAKTA EALDKDGWLH TGDIGKWLPN GTLKIIDRKK HIFKLAQGEY IAPEKIENIY MRSEPVAQVF VHGESLQAFL IAIVVPDVET LCSWAQKRGF EGSFEELCRN KDVKKAILED MVRLGKDSGL KPFEQVKGIT LHPELFSIDN GLLTPTMKAK RPELRNYFRS QIDDLYSTIK V (349-698 aa encoded by BC050073)

Target Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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N⁶-methyladenosine (m⁶A) in 18S rRNA promotes fatty acid metabolism and oncogenic transformation.

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Thioesterase induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation.

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Integrated Metabolomics and Lipidomics Analyses Reveal Metabolic Reprogramming in Human Glioma with IDH1 Mutation.

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PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.

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HBXIP up-regulates ACSL1 through activating transcriptional factor Sp1 in breast cancer.

Biochemical and biophysical research communications

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HBXIP up-regulates ACSL1 through activating transcriptional factor Sp1 in breast cancer.

Biochemical and biophysical research communications

Wang Y,Cai X,Zhang S,Cui M,Liu F,Sun B,Zhang W,Zhang X,Ye L

Sat Mar 11 00:00:00 EST 2017

HBXIP up-regulates ACSL1 through activating transcriptional factor Sp1 in breast cancer.

Biochemical and biophysical research communications

Wang Y,Cai X,Zhang S,Cui M,Liu F,Sun B,Zhang W,Zhang X,Ye L

Sat Mar 11 00:00:00 EST 2017

HBXIP up-regulates ACSL1 through activating transcriptional factor Sp1 in breast cancer.

Biochemical and biophysical research communications

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Sat Mar 11 00:00:00 EST 2017

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Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.

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Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.

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Protective effects of dioscin against alcohol-induced liver injury.

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MiR-205 modulates abnormal lipid metabolism of hepatoma cells via targeting acyl-CoA synthetase long-chain family member 1 (ACSL1) mRNA.

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Evidence-based complementary and alternative medicine : eCAM

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Chinese medicine formula lingguizhugan decoction improves Beta-oxidation and metabolism of Fatty Acid in high-fat-diet-induced rat model of Fatty liver disease.

Evidence-based complementary and alternative medicine : eCAM

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Bioinformatics

Protein Aliases: ACS1; Acyl CoA synthetase, long chain; Acyl-CoA synthetase 1; Arachidonate--CoA ligase; fatty acid Coenzyme A ligase, long chain 2; fatty-acid-Coenzyme A ligase, long-chain 1; fatty-acid-Coenzyme A ligase, long-chain 2; L LACS2; LACS 1; LACS 2; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; long-chain fatty-acid-coenzyme A ligase 1; Long-chain-fatty-acid--CoA ligase 1; Long-chain-fatty-acid--CoA ligase, liver isozyme; OTTHUMP00000218457; OTTHUMP00000218460; OTTHUMP00000218462; OTTHUMP00000218465; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1; Phytanate--CoA ligase

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Gene Aliases: Acas; Acas1; ACS; ACS1; ACSL1; Acsl2; COAA; FACL1; FACL2; FACS; LACS; LACS 1; LACS1; LACS2

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UniProt ID: (Human) Q8TA99, (Mouse) P41216, (Rat) P18163

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Entrez Gene ID: (Human) 2180, (Mouse) 14081, (Rat) 25288

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Function(s)

long-chain fatty acid-CoA ligase activity ATP binding decanoate--CoA ligase activity acetate-CoA ligase activity ligase

Process(es)

long-chain fatty acid metabolic process triglyceride metabolic process response to nutrient lipid biosynthetic process response to organic cyclic compound fatty acid transport adiponectin-activated signaling pathway response to oleic acid long-chain fatty-acyl-CoA biosynthetic process alpha-linolenic acid metabolic process xenobiotic catabolic process response to drug linoleic acid metabolic process long-chain fatty acid import positive regulation of protein serine/threonine kinase activity lipid metabolic process fatty acid metabolic process response to organic substance

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ACSL1 Polyclonal Antibody

ACSL1 Antibody (13989-1-AP) in ICC/IF

Product Details

13989-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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