Proteintech

ACSL1 Polyclonal Antibody

Product Image Gallery

ACSL1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 13989-1-AP (ACSL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ACSL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13989-1-AP ( ACSL1 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ACSL1 Antibody in Western Blot (WB) — Human cerebellum tissue were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ACSL1 Antibody in Immunoprecipitation (IP) — IP result of anti-ACSL1 (IP:13989-1-AP, 4 ug; Detection:13989-1-AP 1:300) with mouse liver tissue lysate 4000 ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:1000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ACSL1 Fusion Protein Ag5059

Conjugate

Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages. It has 2 isoforms produced by alternative splicing.

Target Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ACS1; Acyl CoA synthetase, long chain; Acyl-CoA synthetase 1; Arachidonate--CoA ligase; fatty acid Coenzyme A ligase, long chain 2; fatty-acid-Coenzyme A ligase, long-chain 1; fatty-acid-Coenzyme A ligase, long-chain 2; L LACS2; LACS 1; LACS 2; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; long-chain fatty-acid-coenzyme A ligase 1; Long-chain-fatty-acid--CoA ligase 1; Long-chain-fatty-acid--CoA ligase, liver isozyme; OTTHUMP00000218457; OTTHUMP00000218460; OTTHUMP00000218462; OTTHUMP00000218465; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; paltimoyl-CoA ligase 1; Phytanate--CoA ligase

Gene Aliases: Acas; Acas1; ACS; ACS1; ACSL1; Acsl2; COAA; FACL1; FACL2; FACS; LACS; LACS 1; LACS1; LACS2

UniProt ID: (Human) P33121, (Mouse) P41216, (Rat) P18163

Entrez Gene ID: (Human) 2180, (Mouse) 14081, (Rat) 25288

Molecular Function: ligase