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          • Primary Antibodies ›
          • AKT2 Antibodies

          Invitrogen

          AKT2 Monoclonal Antibody (4H7)

          Advanced Verification

          This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

          View all (59) AKT2 antibodies
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          Cite AKT2 Monoclonal Antibody (4H7)

          Datasheet
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          • Antibody Testing Data (8)
          • Advanced Verification (1)
          AKT2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          AKT2 Antibody (MA1-034) in ICC/IF

          Immunofluorescent analysis of AKT2 (green) showing staining in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an AKT2 monoclonal antibody (Product # MA1-034) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLig... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          MA1-034

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunohistochemistry (IHC)

          1:200
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          -

          Immunoprecipitation (IP)

          2 µg
          -

          ChIP assay (ChIP)

          1-3 µL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          4H7

          Immunogen

          Full length human recombinant protein of human AKT2 (NP_001617) produced in HEK293T cell.
          View immunogen

          Conjugate

          Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          RRID

          AB_11153437

          Target Information

          AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: AKT2 kinase; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; protein kinase B, beta; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; thymoma viral proto-oncogene 2; v-akt murine thymoma viral oncogene homolog 2

          View more View less

          Gene Aliases: 2410016A19Rik; AKT2; AW554154; HIHGHH; PKB; PKBB; PKBBETA; PRKBB; RAC-BETA

          View more View less

          UniProt ID: (Human) P31751, (Rat) P47197, (Mouse) Q60823

          View more View less

          Entrez Gene ID: (Human) 208, (Rat) 25233, (Mouse) 11652

          View more View less

          Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

          Function(s)
          positive regulation of protein phosphorylation glycogen biosynthetic process glucose metabolic process regulation of translation cellular protein modification process apoptotic process response to osmotic stress signal transduction insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction negative regulation of plasma membrane long-chain fatty acid transport positive regulation of glucose metabolic process response to muscle activity peptidyl-serine phosphorylation regulation of cell migration positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance cellular response to insulin stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation intracellular signal transduction negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis mammary gland epithelial cell differentiation intracellular protein transmembrane transport regulation of cell cycle arrest cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus protein phosphorylation multicellular organism development positive regulation of gene expression positive regulation of sodium ion transport response to insulin cellular response to hormone stimulus negative regulation of apoptotic process carbohydrate metabolic process glycogen metabolic process transport phosphorylation
          Process(es)
          protein serine/threonine kinase activity protein kinase C binding protein binding ATP binding protein kinase activity nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups
          It has to be done as per old AB suggested Products section.
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