OriGene

ALDH1L1 Monoclonal Antibody (OTI4C11), TrueMAB™

Product Image Gallery

ALDH1L1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-ALDH1L1 mouse monoclonal antibody. (TA501851)

ALDH1L1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (RC213720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH. Positive lysates LY415919 (100 µg) and LC415919 (20 µg) can be purchased separately from OriGene.

ALDH1L1 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC213720 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH1L1 antibody (TA501851), and then analyzed by flow cytometry.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:100

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI4C11

Immunogen

Full length human recombinant protein of human ALDH1L1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 10-formyltetrahydrofolate dehydrogenase; 10-fTHF; 10-FTHFDH; aldehyde dehydrogenase 1 family, member L1; Aldehyde dehydrogenase family 1 member L1; Cytosolic 10-formyltetrahydrofolate dehydrogenase; FTHFD; MER57B-ALDH1L1

Gene Aliases: 10-fTHF; 10-FTHFDH; ALDH1L1; FDH; FTHFD

UniProt ID: (Human) O75891

Entrez Gene ID: (Human) 10840

Molecular Function: dehydrogenase oxidoreductase