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Immunohistochemistry analysis of ALK showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a ALK polyclonal antibody (513900) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein derived from amino acids 1359-1466 of the 1620 amino acid human ALK protein which corresponds to amino acids 426-528 of the 680 NPM-ALK chimeric protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain.
51-3900 was used in western blot to test if midkine and anaplastic lymphoma kinase expression and signaling are activated by ethanol.
|He D,Chen H,Muramatsu H,Lasek AW||Journal of neurochemistry (135:508)||2015|
A novel mechanism of EML4-ALK rearrangement mediated by chromothripsis in a patient-derived cell line.
51-3900 was used in western blot to examine the mechanism of EML4-ALK rearrangement using a novel cell line
|Kodama T,Motoi N,Ninomiya H,Sakamoto H,Kitada K,Tsukaguchi T,Satoh Y,Nomura K,Nagano H,Ishii N,Terui Y,Hatake K,Ishikawa Y||Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer (9:1638)||2014|
Selective ALK inhibitor alectinib with potent antitumor activity in models of crizotinib resistance.
51-3900 was used in western blot to assess the efficacy of a selective ALK inhibitor alectinib/CH5424802 in models of crizotinib resistance.
|Kodama T,Tsukaguchi T,Yoshida M,Kondoh O,Sakamoto H||Cancer letters (351:215)||2014|
Antineoplastic activity of the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine in anaplastic large cell lymphoma.
51-3900 was used in western blot to investigate the therapeutic use of 5-aza-2'-deoxycytidine on anaplastic large cell lymphoma.
|Hassler MR,Klisaroska A,Kollmann K,Steiner I,Bilban M,Schiefer AI,Sexl V,Egger G||Biochimie (94:2297)||2012|
PDGFR blockade is a rational and effective therapy for NPM-ALK-driven lymphomas.
51-3900 was used in immunohistochemistry - paraffin section to study the therapeutic potential of PDGFR inhibition in anaplastic large cell lymphoma.
|Laimer D,Dolznig H,Kollmann K,Vesely PW,Schlederer M,Merkel O,Schiefer AI,Hassler MR,Heider S,Amenitsch L,Thallinger C,Staber PB,Simonitsch-Klupp I,Artaker M,Lagger S,Turner SD,Pileri S,Piccaluga PP,Valent P,Messana K,Landra I,Weichhart T,Knapp S,Shehata M,Todaro M,Sexl V,Höfler G,Piva R,Medico E,Ruggeri BA,Cheng M,Eferl R,Egger G,Penninger JM,Jaeger U,Moriggl R,Inghirami G,Kenner L||Nature medicine (18:1699)||2012|
Anaplastic lymphoma kinase (ALK) activates Stat3 and protects hematopoietic cells from cell death.
||Zamo A,Chiarle R,Piva R,Howes J,Fan Y,Chilosi M,Levy DE,Inghirami G||Oncogene (21:1038)||2002|
ALK tyrosine kinase receptor, CD246 antigen, mutant anaplastic lymphoma kinase, anaplastic lymphoma kinase, NBLST3, TFG/ALK