Invitrogen

ARF1/ARF2/ARF3/ARF4 Polyclonal Antibody

View all (3) ARF Pan antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-18627

Applications
Tested Dilution
Publications

Western Blot (WB)

0.3-1 µg/mL
-

Flow Cytometry (Flow)

10 µg/mL
-
Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide sequence (EGLDWLSNQLRNQK) corresponding to the C-terminus amino acids of ARF1 (aa 168-181).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981429

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.

Target Information

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 5; ADP-ribosylation factor 6; ARF; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein

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Gene Aliases: 5430400P17Rik; AA407803; Ac1-253; AI788669; AI854770; ARF1; ARF2; ARF3; ARF4; ARF5; ARF6; AW496366

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UniProt ID: (Human) P84077, (Human) P61204, (Human) P18085, (Human) P84085, (Human) P62330, (Mouse) P84078, (Rat) P84079, (Mouse) Q8BSL7, (Rat) P84082, (Rat) P61206, (Mouse) P61205, (Rat) P61751, (Mouse) P61750, (Mouse) P84084, (Rat) P84083, (Mouse) P62331, (Rat) P62332

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Entrez Gene ID: (Dog) 100856517, (Human) 375, (Dog) 486562, (Human) 377, (Dog) 607073, (Human) 378, (Dog) 475206, (Human) 381, (Human) 382, (Dog) 490679, (Mouse) 11840, (Rat) 64310, (Mouse) 11841, (Rat) 79119, (Rat) 140940, (Mouse) 11842, (Rat) 79120, (Mouse) 11843, (Mouse) 11844, (Rat) 79117, (Mouse) 11845, (Rat) 79121

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Function(s)
magnesium ion binding GTPase activity receptor signaling protein activity protein binding GTP binding GDP binding poly(A) RNA binding phospholipase D activator activity ARF guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding thioesterase binding protein N-terminus binding nucleotide binding protein homodimerization activity G-protein
Process(es)
regulation of receptor internalization phosphatidylinositol biosynthetic process cellular copper ion homeostasis post-Golgi vesicle-mediated transport actin filament organization small GTPase mediated signal transduction protein transport antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of Arp2/3 complex-mediated actin nucleation very-low-density lipoprotein particle assembly positive regulation of catalytic activity positive regulation of endocytosis positive regulation of calcium ion-dependent exocytosis regulation of defense response to virus by virus positive regulation of protein secretion Golgi to transport vesicle transport long term synaptic depression positive regulation of dendritic spine development synaptic vesicle budding dendritic spine organization lysosomal membrane organization positive regulation of sodium ion transmembrane transport positive regulation of late endosome to lysosome transport positive regulation of ER to Golgi vesicle-mediated transport regulation of phospholipid metabolic process retrograde vesicle-mediated transport, Golgi to ER protein ADP-ribosylation ER to Golgi vesicle-mediated transport epidermal growth factor receptor signaling pathway brain development learning cell migration activation of phospholipase D activity negative regulation of apoptotic process positive regulation of GTPase activity apical protein localization establishment or maintenance of epithelial cell apical/basal polarity positive regulation of transcription from RNA polymerase II promoter response to axon injury dendritic spine development protein localization to cilium regulation of reactive oxygen species metabolic process liver development movement of cell or subcellular component cell cycle cell adhesion vesicle-mediated transport positive regulation of actin filament polymerization cortical actin cytoskeleton organization ruffle organization myeloid cell apoptotic process protein localization to cell surface regulation of Rac protein signal transduction protein localization to endosome negative regulation of receptor-mediated endocytosis cell division regulation of filopodium assembly regulation of dendritic spine development positive regulation of establishment of protein localization to plasma membrane establishment of epithelial cell polarity hepatocyte apoptotic process negative regulation of dendrite development transport nervous system development cell differentiation regulation of toll-like receptor 4 signaling pathway actin cytoskeleton organization
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