Invitrogen

AS160 Monoclonal Antibody (G.853.7)

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AS160 Antibody in Western Blot (WB) — Western blot was performed using Anti-AS160 Monoclonal Antibody (G.853.7) (Product # MA5-14840) and a 146 kDa band corresponding to AS160 was observed in cell lines tested. Whole cell extracts (40 µg lysate) of Caco-2 (Lane 1) and HEK-293 (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

MA5-14840

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 2 publications 2 publications
Immunoprecipitation (IP)	1:50	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Monoclonal
Type	Antibody
Clone	G.853.7
Immunogen	Synthetic peptide corresponding to residues surrounding Ala195 of human AS160 View antigen
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	<0.02% sodium azide
Storage conditions	-20°C
RRID	AB_10979793

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

AS160 (TBC1D4) is a GTPase-activiting protein for RAB2A, RAB8A, RAB10, and RAB14. The isoform 2 version of this protein promotes the insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane. This movement increases glucose uptake. Mutations associated with the gene cause Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Zuliani I,Lanzillotta C,Tramutola A,Francioso A,Pagnotta S,Barone E,Perluigi M,Di Domenico F

MA5-14840 was used in Western Blotting to determine the role of protein O-GlcNacylation in the down syndrome brain.

Fri Jan 01 00:00:00 EST 2021

Adipose Tissue Insulin Resistance in Gestational Diabetes.

Metabolic syndrome and related disorders

Tumurbaatar B,Poole AT,Olson G,Makhlouf M,Sallam HS,Thukuntla S,Kankanala S,Ekhaese O,Gomez G,Chandalia M,Abate N

Published figure using AS160 monoclonal antibody (Product # MA5-14840) in Western Blot

Wed Mar 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Akt substrate of 160 kDa; AS160; DKFZp779C0666; OTTHUMP00000040818; RP11-159J2.1; RP11-159J2.3; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC Tre-2; TBC1 domain family member 4; TBC1 domain family, member 4

Gene Aliases: 5930406J04Rik; A930035N22; AS160; AV295684; KIAA0603; NIDDM5; RGD1561609; TBC1D4

UniProt ID: (Human) O60343, (Mouse) Q8BYJ6

Entrez Gene ID: (Human) 9882, (Rat) 306117, (Mouse) 210789

Molecular Function: GTPase-activating protein scaffold/adaptor protein