Invitrogen

AS160 Monoclonal Antibody (G.853.7)

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AS160 Antibody in Western Blot (WB) — Western blot was performed using Anti-AS160 Monoclonal Antibody (G.853.7) (Product # MA5-14840) and a 146 kDa band corresponding to AS160 was observed in cell lines tested. Whole cell extracts (40 µg lysate) of Caco-2 (Lane 1) and HEK-293 (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

MA5-14840

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunoprecipitation (IP)

1:50

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

G.853.7

Immunogen

Synthetic peptide corresponding to residues surrounding Ala195 of human AS160

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

81 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10979793

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

AS160 (TBC1D4) is a GTPase-activiting protein for RAB2A, RAB8A, RAB10, and RAB14. The isoform 2 version of this protein promotes the insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane. This movement increases glucose uptake. Mutations associated with the gene cause Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Dysregulation of OGT/OGA Cycle Mediates Tau and APP Neuropathology in Down Syndrome.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Zuliani I,Lanzillotta C,Tramutola A,Francioso A,Pagnotta S,Barone E,Perluigi M,Di Domenico F

MA5-14840 was used in Western Blotting to determine the role of protein O-GlcNacylation in the down syndrome brain.

Fri Jan 01 00:00:00 EST 2021

Adipose Tissue Insulin Resistance in Gestational Diabetes.

Metabolic syndrome and related disorders

Tumurbaatar B,Poole AT,Olson G,Makhlouf M,Sallam HS,Thukuntla S,Kankanala S,Ekhaese O,Gomez G,Chandalia M,Abate N

Published figure using AS160 monoclonal antibody (Product # MA5-14840) in Western Blot

Wed Mar 01 00:00:00 EST 2017

Bioinformatics

Protein Aliases: Akt substrate of 160 kDa; AS160; DKFZp779C0666; OTTHUMP00000040818; RP11-159J2.1; RP11-159J2.3; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC Tre-2; TBC1 domain family member 4; TBC1 domain family, member 4

Gene Aliases: 5930406J04Rik; A930035N22; AS160; AV295684; KIAA0603; NIDDM5; RGD1561609; TBC1D4

UniProt ID: (Human) O60343, (Mouse) Q8BYJ6

Entrez Gene ID: (Human) 9882, (Rat) 306117, (Mouse) 210789

Molecular Function: GTPase-activating protein scaffold/adaptor protein