|Tested species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Bovine Complex V|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/ml|
|Immunocytochemistry (ICC)||1-5 µg/ml|
|Immunofluorescence (IF)||1-5 µg/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For mouse and rat samples, this antibody will only recognize ATP5H in purified mitochondrial samples. Mouse and rat cell lysates and tissue homogenates are not recommended with this antibody.
For positive control, use Isolated mitochondria from Human heart, Bovine heart, Rat heart, Mouse heart, and HepG2, Cultured Human embryonic lung-derived fibroblasts (strain MRC5), HeLa cells.
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease.
459000 was used in immunocytochemistry to elucidate enrichment of oxidized mitochondrial DNA in neutrophil extracellular traps contributes to lupus-like disease
|Lood C,Blanco LP,Purmalek MM,Carmona-Rivera C,De Ravin SS,Smith CK,Malech HL,Ledbetter JA,Elkon KB,Kaplan MJ||Nature medicine (22:146)||2016|
Population of ATP synthase molecules in mitochondria is limited by available 6.8-kDa proteolipid protein (MLQ).
459000 was used in immunocytochemistry to investigate MLQ as a regulator of mitochondrial ATP synthesis.
|Fujikawa M,Ohsakaya S,Sugawara K,Yoshida M||Genes to cells : devoted to molecular and cellular mechanisms (19:153)||2014|
Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex.
459000 was used in western blot to study the assembly process of human mitochondrial ATP synthase
|Fujikawa M,Sugawara K,Tanabe T,Yoshida M||FEBS letters (589:2707)||2015|