Invitrogen

Actin Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (23) Actin antibodies

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Datasheet

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FIGURE: 1 / 8

Actin Antibody (PA5-78715) in WB

Western blot was performed using Actin Polyclonal Antibody (Product # PA5-78715) and a 40 kDa band corresponding to Actin was observed across cell lines and tissue extracts along with an uncharacterized band (*) at ~120 kDa in the tissue extract tested. Whole cell extracts (30 µg lysate) of Rat Heart (Lane 1), Rat Skeletal Muscle (Lane 2), Rat Kidney (Lane 3), Mouse Heart (Lane 4), Mouse Skeletal Muscle (Lane 5) and Mouse Kidney (Lane 6) of Fig. a; cell lines of C2C12 (Lane 1), L6 (Lane 2), Raji (Lane 3) and Hep G2 (Lane 4) from Fig. b wer... View More

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Actin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Actin on paraffin-embedded human mammary cancer tissue. Sample was incubated with Actin polyclonal antibody (Product# PA5-78715).

Actin Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of Actin was observed in Rat Heart, Rat Skeletal Muscle, Mouse Heart and Mouse Skeletal Muscle in comparison to Mouse Kidney and Rat Kidney using Actin Polyclonal Antibody (Product # PA5-78715) in Western Blot. {RE}

Product Details

PA5-78715

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Actin (277-308aa HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY).

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

RRID

AB_2745831

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. In vertebrates there are three groups of actin isoforms: alpha, beta and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exists in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Mechanisms of FH Protection Against Neovascular AMD.

Frontiers in immunology

Borras C,Delaunay K,Slaoui Y,Abache T,Jorieux S,Naud MC,Sanharawi ME,Gelize E,Lassiaz P,An N,Kowalczuk L,Ayassami C,Moulin A,Behar-Cohen F,Mascarelli F,Dinet V

Published figure using Actin polyclonal antibody (Product # PA5-78715) in Western Blot

Fri Mar 19 00:00:00 EDT 2021

Mechanisms of FH Protection Against Neovascular AMD.

Frontiers in immunology

Borras C,Delaunay K,Slaoui Y,Abache T,Jorieux S,Naud MC,Sanharawi ME,Gelize E,Lassiaz P,An N,Kowalczuk L,Ayassami C,Moulin A,Behar-Cohen F,Mascarelli F,Dinet V

Published figure using Actin polyclonal antibody (Product # PA5-78715) in Western Blot

Fri Mar 19 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: actin alpha 1; actin alpha cardiac; actin alpha cardiac 1; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; Actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, vascular smooth muscle; Actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; actin, gamma, cytoplasmic 1; Actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; alpha-actin cardiac; Alpha-actin-1; Alpha-actin-2; Alpha-actin-3; Alpha-cardiac actin; alpha-sma; alpha-smooth muscle actin; alphac-actin; Atcb; beta cytoskeletal actin; Beta-actin; beta-actin FE-3; Cell growth-inhibiting gene 46 protein; cytoplasmic beta-actin; cytoskeletal gamma-actin; deafness, autosomal dominant 20; deafness, autosomal dominant 26; epididymis luminal protein 176; Gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; nemaline myopathy type 3; pan actin; PS1TP5-binding protein 1; RP5-1068B5.2; smooth muscle alpha-actin; Smooth muscle gamma-actin

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Gene Aliases: 0610041G09Rik; a-SMA; AA959943; AAT6; ACT; Act-4; Act4; ACTA; Acta-2; ACTA1; ACTA2; ACTA3; ACTB; ACTC; Actc-1; ACTC1; ACTE; ACTG; ACTG1; ACTG2; ACTGE; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; ACTVS; Actx; AL023024; alphaSMA; ASD5; ASMA; beta-actin; BRWS1; BRWS2; CFTD; CFTD1; CFTDM; CMD1R; CMH11; DFNA20; DFNA26; E430023M04Rik; E51; GIG46; HEL-176; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; PS1TP5BP1; SHPM; SMalphaA; SMGA; VSCM

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Entrez Gene ID: (Human) 58, (Human) 59, (Human) 60, (Human) 70, (Human) 71, (Human) 72, (Mouse) 11459, (Rat) 29437, (Rat) 81633, (Mouse) 11475, (Rat) 81822, (Mouse) 11461, (Mouse) 11464, (Rat) 29275, (Mouse) 11465, (Rat) 287876, (Mouse) 11468, (Rat) 25365

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Molecular Function: actin and actin related protein actin or actin-binding cytoskeletal protein cytoskeletal protein

Function(s)

muscle contraction response to mechanical stimulus response to extracellular stimulus response to lithium ion positive regulation of gene expression cell growth muscle filament sliding skeletal muscle thin filament assembly skeletal muscle fiber adaptation response to steroid hormone skeletal muscle fiber development mesenchyme migration regulation of blood pressure response to virus vascular smooth muscle contraction glomerular mesangial cell development retina homeostasis movement of cell or subcellular component cytoskeleton organization substantia nigra development cell junction assembly Fc-gamma receptor signaling pathway involved in phagocytosis ATP-dependent chromatin remodeling positive regulation of gene expression, epigenetic vascular endothelial growth factor receptor signaling pathway ephrin receptor signaling pathway platelet aggregation apoptotic process actin filament-based movement actomyosin structure organization actin-myosin filament sliding response to drug negative regulation of apoptotic process response to ethanol cardiac myofibril assembly cardiac muscle tissue morphogenesis heart contraction cardiac muscle contraction sarcomere organization axonogenesis retina development in camera-type eye cellular response to electrical stimulus metabolic process actin-mediated cell contraction response to calcium ion biological_process

Process(es)

structural constituent of cytoskeleton protein binding ATP binding myosin binding ADP binding protein kinase binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding kinesin binding Tat protein binding nucleosomal DNA binding identical protein binding nitric-oxide synthase binding ATPase activity ubiquitin protein ligase binding nucleotide binding molecular_function

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Actin Polyclonal Antibody

Actin Antibody (PA5-78715) in WB

Product Details

PA5-78715

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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