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Adenosine Deaminase Antibody (PA5-78722) in IHC (P)
Immunohistochemistry analysis of ADA on paraffin-embedded mouse intestine tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with ADA polyclonal antibody (Product# PA5-78722) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Developm... View More
Product Details
PA5-78722
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived rat ADA recombinant protein (Position: A2-H241).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745838
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, rat liver tissue, rat liver tissue, mouse liver tissue, mouse spleen tissue, mouse NIH/3T3 whole cell. IHC: rat spleen tissue, mouse intestine tissue, rat brain tissue.
Target Information
Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. May act as a positive regulator of T-cell coactivation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenosine aminohydrolase; Adenosine deaminase; RP11-61L14.5
Gene Aliases: Ada
UniProt ID: (Mouse) P03958, (Rat) Q920P6
Entrez Gene ID: (Mouse) 11486, (Rat) 24165
response to hypoxia
in utero embryonic development
histamine secretion
trophectodermal cell differentiation
liver development
placenta development
germinal center B cell differentiation
positive regulation of germinal center formation
negative regulation of leukocyte migration
negative regulation of mature B cell apoptotic process
adenosine catabolic process
deoxyadenosine catabolic process
cell adhesion
nucleotide metabolic process
purine ribonucleoside monophosphate biosynthetic process
positive regulation of heart rate
lung development
positive regulation of B cell proliferation
purine nucleotide salvage
positive regulation of T cell differentiation in thymus
response to vitamin E
regulation of cell-cell adhesion mediated by integrin
T cell activation
negative regulation of circadian sleep/wake cycle, non-REM sleep
negative regulation of apoptotic process
hypoxanthine salvage
response to morphine
regulation of T cell differentiation
positive regulation of T cell differentiation
positive regulation of smooth muscle contraction
dATP catabolic process
adenosine metabolic process
hypoxanthine biosynthetic process
inosine biosynthetic process
xanthine biosynthetic process
positive regulation of alpha-beta T cell differentiation
lung alveolus development
Peyer's patch development
embryonic digestive tract development
negative regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
negative regulation of adenosine receptor signaling pathway
negative regulation of penile erection
negative regulation of thymocyte apoptotic process
negative regulation of mucus secretion
aging
response to drug
response to hydrogen peroxide
regulation of circadian sleep/wake cycle, sleep
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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