Immunofluorescent analysis of Adrenocorticotropic Hormone in AtT-20 cells using an Adrenocorticotropic Hormone polyclonal antibody (Product # PA1-29875) at a dilution of 1:200 followed by detection using a Donkey anti-IgY Fc-FITC secondary antibody at a dilution of 1:200.
|Tested species reactivity||Mouse, Human, Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Recombinant fragment, corresponding to amino acids 6-266 of Human ACTH|
|Purification||Antigen affinity chromatography|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29875 detects ACTH from human, mouse, and rat samples.
PA1-29875 has been successfully used in immunocytochemistry, and Western blot applications. Predicted molecular weight of 29 kDa.
The PA1-29875 immunogen is recombinant fragment, corresponding to amino acids 6-266 of Human ACTH.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thawe cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.