Invitrogen

Aldolase A Recombinant Rabbit Monoclonal Antibody (21H7L6)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Aldolase A Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous Aldolase using Anti- Aldolase Recombinant Rabbit Monoclonal Antibody (Product # 702729, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of Aldolase protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Aldolase A. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Aldolase A Antibody in Western Blot (WB) — Western blot analysis was performed on Whole cell extracts (30 µg lysate) of A549 (Lane 1), NCI-H1975 (Lane 2), Hep G2 (Lane 3), PANC-1 (Lane 4), SW480 (Lane 5), L6 (Lane 6), C2C12 (Lane 7) and tissue extracts of Mouse Skeletal Muscle (Lane 8). The blots were probed with Anti-Aldolase A Recombinant Rabbit Monoclonal Antibody (Product # 702729, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 40 kDa band corresponding to Aldolase A was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Aldolase A Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inAldolase A KO cell line compared to control cell line using Anti-Aldolase A Recombinant Rabbit Monoclonal Antibody (21H7L6) (Product # 702729). {KO}

Product Details

702729

Applications	Tested Dilution	Publications
Western Blot (WB)	2.5 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Expression System	EXPI293
Class	Recombinant Monoclonal
Type	Antibody
Clone	21H7L6
Immunogen	Peptides corresponding to human Aldolase A [1) aa341-aa360 2) aa84-aa103] View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2722843

Product Specific Information

This antibody is predicted to react with Monkey, Cat, Rabbit

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Aldolase encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aldolase 1; aldolase 1, A isoform; aldolase A, fructose-bisphosphate; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase

Gene Aliases: ALDA; Aldo-1; Aldo1; ALDOA; GSD12; HEL-S-87p; RNALDOG5

UniProt ID: (Human) P04075, (Mouse) P05064, (Rat) P05065

Entrez Gene ID: (Human) 226, (Mouse) 11674, (Rat) 24189

Molecular Function: aldolase