|Tested species reactivity||Human|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 124 of Human Amphiregulin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-27298 targets Amphiregulin in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27298 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 124 of Human Amphiregulin.
The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells, and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). This protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells and inhibits the growth of certain aggressive carcinoma cell lines. This encoded protein is associated with a psoriasis-like skin phenotype.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amphiregulin activates human hepatic stellate cells and is upregulated in non alcoholic steatohepatitis.
PA5-27298 was used in western blot to examine the effect of amphiregulin on human hepatic stellate cells ex vivo and verify the contribution of amphiregulin to non-alcoholic fatty liver disease fibrogenesis in vivo.
|McKee C,Sigala B,Soeda J,Mouralidarane A,Morgan M,Mazzoccoli G,Rappa F,Cappello F,Cabibi D,Pazienza V,Selden C,Roskams T,Vinciguerra M,Oben JA||Scientific reports (5:null)||2015|