Invitrogen

Annexin A1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (69) Annexin A1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Annexin A1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

Annexin A1 Antibody (PA5-27315) in ICC/IF

Annexin A1 Polyclonal Antibody detects Annexin A1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Blue: Fluoroshield with DAPI . Scale bar= 10 µm.

Product Image Gallery

Annexin A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Annexin A1 Polyclonal Antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Annexin A1 stained by Annexin A1 Polyclonal Antibody (Product # PA5-27315) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Annexin A1 Antibody in Western Blot (WB) — Western Blot analysis of Annexin A1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Annexin A1 Polyclonal Antibody (Product # PA5-27315) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Annexin A1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Annexin A1 was performed in HeLa whole cell extracts using 5 µg of Annexin A1 Polyclonal Antibody (Product # PA5-27315). Samples were transferred to a membrane and probed with Annexin A1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Annexin A1 Antibody — Antibody specificity was demonstrated by knocking out the target protein. Annexin A1 knockout (KO) HeLa cell extracts show decrease in signal in Western blot application using Annexin A1 Polyclonal Antibody (Product # PA5-27315). {KO}

Product Details

PA5-27315

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 24 and 346 of Human Annexin A1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.95 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544791

Product Specific Information

Recommended positive controls: HeLa, NIH-3T3, JC, ANXA1-transfected 293T.

Predicted reactivity: Mouse (86%), Rat (88%), Dog (91%), Pig (88%), Rabbit (91%), Chimpanzee (100%), Bovine (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Annexin I belongs to a family of Ca (2+) -dependent phospholipid binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Annexin I has a molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Plasma exosomal miR-122 regulates the efficacy of metformin via AMPK in type 2 diabetes and hepatocellular carcinoma.

Heliyon

Peng H,Hou M,Wu Z,Wang J,Zhou M,Zhuang X,Xing J,Tao Q,Huang L,Zhou F,Zhang S,Feng Q,Hou Y,Yu Q

PA5-27315 was used in Western Blotting to reveal a key regulatory role of plasma exosomal miR-122 on the molecular mechanism of metformin.

Tue Nov 01 00:00:00 EDT 2022

Plasma exosomal miR-122 regulates the efficacy of metformin via AMPK in type 2 diabetes and hepatocellular carcinoma.

Heliyon

Peng H,Hou M,Wu Z,Wang J,Zhou M,Zhuang X,Xing J,Tao Q,Huang L,Zhou F,Zhang S,Feng Q,Hou Y,Yu Q

PA5-27315 was used in Western Blotting to reveal a key regulatory role of plasma exosomal miR-122 on the molecular mechanism of metformin.

Tue Nov 01 00:00:00 EDT 2022

Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway.

Journal of neuroinflammation

Xu X,Gao W,Li L,Hao J,Yang B,Wang T,Li L,Bai X,Li F,Ren H,Zhang M,Zhang L,Wang J,Wang D,Zhang J,Jiao L

PA5-27315 was used in Western Blot to report for the first time that the decreased ANXA1 in patients with AIS were recovered 2nd–3rd after successful recanalization by EVT.

Sat May 22 00:00:00 EDT 2021

Lineage Reversion Drives WNT Independence in Intestinal Cancer.

Cancer discovery

Han T,Goswami S,Hu Y,Tang F,Zafra MP,Murphy C,Cao Z,Poirier JT,Khurana E,Elemento O,Hechtman JF,Ganesh K,Yaeger R,Dow LE

PA5-27315 was used in Immunohistochemistry to show that the accumulation of colorectal cancer-associated genetic alterations enables drug resistance and WNT-independent growth.

Thu Oct 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: ANAX1; Annexin 1 (p35) (Lipocortin 1); Annexin A1; Annexin Ac2-26; Annexin I; annexin I (lipocortin I); Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; lipocortin 1; Lipocortin I; p35; Phospholipase A2 inhibitory protein; RP11-71A24.1

Gene Aliases: Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35

UniProt ID: (Human) P04083, (Mouse) P10107, (Rat) P07150

Entrez Gene ID: (Human) 301, (Mouse) 16952, (Rat) 25380

Molecular Function: calcium-binding protein

Function(s)

Process(es)

single-stranded DNA binding single-stranded RNA binding helicase activity receptor binding structural molecule activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding phospholipase A2 inhibitor activity protein binding, bridging double-stranded DNA-dependent ATPase activity annealing helicase activity protein homodimerization activity calcium-dependent protein binding cadherin binding involved in cell-cell adhesion phospholipase inhibitor activity