Invitrogen

Annexin A1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by KnockOut to ensure that the antibody binds to the antigen stated. View Details

View all (19) Annexin A1 antibodies

Datasheet

Datasheet

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Annexin A1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Annexin A1 in paraformaldehyde-fixed HeLa cells using an Annexin A1 polyclonal antibody (Product # PA5-27315) at a 1:200 dilution.

Annexin A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Annexin A1 in the cytosol and nucleus of paraffin-embedded mouse lung using an Annexin A1 polyclonal antibody (Product # PA5-27315) at a dilution of 1:500.

Annexin A1 Antibody in Western Blot (WB) — Western blot analysis of Annexin A1 using 30 µg of A) A431 (B) H1299 and C) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Annexin A1 polyclonal antibody (Product # PA5-27315) at a dilution of 1:1000.

Annexin A1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of Annexin A1 protein from HeLa whole cell extracts using 5 µg of Annexin A1 antibody (Product # PA5-27315).

Annexin A1 Antibody in KnockOut — Antibody specificity was demonstrated by knocking out the target protein. Annexin A1 knockout (KO) HeLa cell extracts show decrease in signal in Western blot application using Annexin A1 Polyclonal Antibody (Product # PA5-27315). {KO}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

1:1000-1:10,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 24 and 346 of Human Annexin A1

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544791

Product Specific Information

PA5-27315 targets Annexin A1 in IF, IHC (P), and WB applications and shows reactivity with Human, rat and mouse samples.

The PA5-27315 immunogen is recombinant fragment corresponding to a region within amino acids 24 and 346 of Human Annexin A1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Annexin 1 (p35) (Lipocortin 1); Annexin A1; Annexin I; annexin I (lipocortin I); Annexin-1; ANX1; Calpactin II; Calpactin-2; Chromobindin-9; lipocortin 1; Lipocortin I; LPC1; p35; Phospholipase A2 inhibitory protein

Gene Aliases: Anx-1; Anx-A1; ANX1; ANXA1; C430014K04Rik; Lpc-1; LPC1; p35

UniProt ID: (Human) P04083, (Mouse) P10107, (Rat) P07150

Entrez Gene ID: (Human) 301, (Mouse) 16952, (Rat) 25380

Function(s)