Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:10,000 | View 19 publications 19 publications |
Immunohistochemistry (IHC) |
- | View 3 publications 3 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:400-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:500 | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Mouse / IgG2b |
Class |
Monoclonal |
Type |
Antibody |
Clone |
4G5E8 |
Immunogen |
BAX Fusion Protein Ag21068 (1-192 aa encoded by BC014175) |
Conjugate |
Unconjugated
|
Form |
Liquid |
Concentration |
1.6 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MDGSGEQPR GGGPTSSEQI MKTGALLLQG FIQDRAGRMG GEAPELALDP VPQDASTKKL SECLKRIGDE LDSNMELQRM IAAVDTDSPR EVFFRVAADM FSDGNFNWGR VVALFYFASK LVLKALCTKV PELIRTIMGW TLDFLRERLL GWIQDQGGWD GLLSYFGTPT WQTVTIFVAG VLTASLTIWK KMG (1-192 aa encoded by BC014175)
BAX is a members of the Bcl-2 Family and plays an important role in regulation of apoptosis. Whereas Bcl-2 is commonly regarded as an anti-apoptotic protein, BAX is considered to have a pro-apoptotic function. Regulation of apoptosis is supposed to involve both homo- and heterodimerization of different isoforms of BAX and Bcl-2. The Bax gene encodes different isoforms including Bax alpha (21 kDa) and Bax beta (24 kDa), whereas both isoforms contain the BH1, BH2 and BH3 domains, Bax beta has a unique carboxyl terminus and does not contain a hydrophobic transmembrane domain. Bcl-2 is also expressed in different Isoforms. Bcl-2 beta differs in the 3' UTR and coding region compared to variant alpha. Bcl-2 beta is shorter (22 kDa) and has a distinct C-terminus compared to Bcl-2 alpha (26 kDa). BAX is a member of the BCL-2 family of proteins, which function as regulators of apoptosis. Overexpression of BAX functions to promote cell death. BAX can form homodimers and is also able to heterodimerize with other BCL-2 related proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Apoptosis regulator BAX; Bax zeta; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; Bcl-2-like protein 4; BCL2 associated X protein; BCL2-associated X protein omega; Bcl2-L-4
Gene Aliases: BAX; BCL2L4
UniProt ID: (Human) Q07812, (Mouse) Q07813
Entrez Gene ID: (Human) 581, (Rat) 24887, (Mouse) 12028
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