Proteintech

BMI1 Monoclonal Antibody (2E10A11)

BMI1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

BMI1 Antibody (66161-1-IG) in ICC/IF

Immunofluorescent analysis of COS-7 cells using 66161-1-IG (BMI1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IGG.

BMI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

BMI1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

66161-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2E10A11

Immunogen

BMI1 Fusion Protein Ag21284 (1-326 aa encoded by BC011652)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.57 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody reacts with the 37 kDa BMI1 protein.

Immunogen sequence: MHRTTRIKI TELNPHLMCV LCGGYFIDAT TIIECLHSFC KTCIVRYLET SKYCPICDVQ VHKTRPLLNI RSDKTLQDIV YKLVPGLFKN EMKRRRDFYA AHPSADAANG SNEDRGEVAD EDKRIITDDE IISLSIEFFD QNRLDRKVNK DKEKSKEEVN DKRYLRCPAA MTVMHLRKFL RSKMDIPNTF QIDVMYEEEP LKDYYTLMDI AYIYTWRRNG PLPLKYRVRP TCKRMKISHQ RDGLTNAGEL ESDSGSDKAN SPAGGIPSTS SCLPSPSTPV QSPHPQFPHI SSTMNGTSNS PSGNHQSSFA NRPRKSSVNG SSATSSG (1-326 aa encoded by BC011652)

Target Information

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Apo- and holo-transferrin differentially interact with hephaestin and ferroportin in a novel mechanism of cellular iron release regulation.

Journal of biomedical science

Baringer SL,Palsa K,Spiegelman VS,Simpson IA,Connor JR

Tue Jun 06 00:00:00 UTC 2023

Hairy gene homolog increases nasopharyngeal carcinoma cell stemness by upregulating Bmi-1.

Aging

Lei Y,Shen HF,Li QW,Yang S,Xie HT,Li XF,Chen ML,Xia JW,Wang SC,Dai GQ,Zhou Y,Li YC,Huang SH,He DH,Zhou ZH,Cong JG,Lin XL,Lin TY,Wu AB,Xiao D,Xiao SJ,Zhang XK,Jia JS

Mon May 22 00:00:00 UTC 2023

Hairy gene homolog increases nasopharyngeal carcinoma cell stemness by upregulating Bmi-1.

Aging

Lei Y,Shen HF,Li QW,Yang S,Xie HT,Li XF,Chen ML,Xia JW,Wang SC,Dai GQ,Zhou Y,Li YC,Huang SH,He DH,Zhou ZH,Cong JG,Lin XL,Lin TY,Wu AB,Xiao D,Xiao SJ,Zhang XK,Jia JS

Mon May 22 00:00:00 UTC 2023

Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)₂ D₃ Deficiency and Downregulates GATA4-Dependent Rela Transcription.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Q,Zhao J,Chen H,Zhou J,Chen A,Zhang J,Wang Y,Mao Z,Wang J,Qiu X,Chen Y,Wang R,Zhang Y,Miao D,Jin J

Wed Mar 01 00:00:00 UTC 2023

Chronic Alcohol Reduces Bone Mass Through Inhibiting Proliferation and Promoting Aging of Endothelial Cells in Type-H Vessels.

Stem cells and development

Chen A,Li X,Zhao J,Zhou J,Xie C,Chen H,Wang Q,Wang R,Miao D,Li J,Jin J

Thu Sep 01 00:00:00 UTC 2022

Chronic Alcohol Reduces Bone Mass Through Inhibiting Proliferation and Promoting Aging of Endothelial Cells in Type-H Vessels.

Stem cells and development

Chen A,Li X,Zhao J,Zhou J,Xie C,Chen H,Wang Q,Wang R,Miao D,Li J,Jin J

Thu Sep 01 00:00:00 UTC 2022

Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.

Clinical and translational medicine

Chen H,Zhou J,Chen H,Liang J,Xie C,Gu X,Wang R,Mao Z,Zhang Y,Li Q,Zuo G,Miao D,Jin J

Fri Apr 01 00:00:00 UTC 2022

EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.

Experimental cell research

Tan M,Meng J,Sun X,Fu X,Wang R

Fri Oct 01 00:00:00 UTC 2021

EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.

Experimental cell research

Tan M,Meng J,Sun X,Fu X,Wang R

Fri Oct 01 00:00:00 UTC 2021

Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.

Aging

Zhang Z,Chen J,Huo X,Zong G,Huang K,Cheng M,Sun L,Yue X,Bian E,Zhao B

Mon Apr 19 00:00:00 UTC 2021

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Cancer science

Qiu GZ,Mao XY,Ma Y,Gao XC,Wang Z,Jin MZ,Sun W,Zou YX,Lin J,Fu HL,Jin WL

Sun Jul 01 00:00:00 UTC 2018

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Cancer science

Qiu GZ,Mao XY,Ma Y,Gao XC,Wang Z,Jin MZ,Sun W,Zou YX,Lin J,Fu HL,Jin WL

Sun Jul 01 00:00:00 UTC 2018

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Cancer science

Qiu GZ,Mao XY,Ma Y,Gao XC,Wang Z,Jin MZ,Sun W,Zou YX,Lin J,Fu HL,Jin WL

Sun Jul 01 00:00:00 UTC 2018

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Cancer science

Qiu GZ,Mao XY,Ma Y,Gao XC,Wang Z,Jin MZ,Sun W,Zou YX,Lin J,Fu HL,Jin WL

Sun Jul 01 00:00:00 UTC 2018

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Molecular cancer therapeutics

Dey A,Xiong X,Crim A,Dwivedi SKD,Mustafi SB,Mukherjee P,Cao L,Sydorenko N,Baiazitov R,Moon YC,Dumble M,Davis T,Bhattacharya R

Mon Jan 01 00:00:00 UTC 2018

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Molecular cancer therapeutics

Dey A,Xiong X,Crim A,Dwivedi SKD,Mustafi SB,Mukherjee P,Cao L,Sydorenko N,Baiazitov R,Moon YC,Dumble M,Davis T,Bhattacharya R

Mon Jan 01 00:00:00 UTC 2018

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Molecular cancer therapeutics

Dey A,Xiong X,Crim A,Dwivedi SKD,Mustafi SB,Mukherjee P,Cao L,Sydorenko N,Baiazitov R,Moon YC,Dumble M,Davis T,Bhattacharya R

Mon Jan 01 00:00:00 UTC 2018

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Molecular cancer therapeutics

Dey A,Xiong X,Crim A,Dwivedi SKD,Mustafi SB,Mukherjee P,Cao L,Sydorenko N,Baiazitov R,Moon YC,Dumble M,Davis T,Bhattacharya R

Mon Jan 01 00:00:00 UTC 2018

Inhibition of BMI1 induces autophagy-mediated necroptosis.

Autophagy

Dey A,Mustafi SB,Saha S,Kumar Dhar Dwivedi S,Mukherjee P,Bhattacharya R

Mon Dec 26 00:00:00 UTC 2016

Inhibition of BMI1 induces autophagy-mediated necroptosis.

Autophagy

Dey A,Mustafi SB,Saha S,Kumar Dhar Dwivedi S,Mukherjee P,Bhattacharya R

Mon Dec 26 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: B lymphoma Mo-MLV insertion region 1; B lymphoma Mo-MLV insertion region 1 homolog; BMI1 polycomb ring finger oncogene; BMI1 polycomb ring finger proto-oncogene; murine leukemia viral (bmi-1) oncogene homolog; Pcgf4 antibody; Polycomb complex protein BMI-1; polycomb group protein Bmi1; polycomb group ring finger 4; Polycomb group RING finger protein 4; RING finger protein 51; RP11-573G6.1

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Gene Aliases: AW546694; Bmi-1; BMI1; flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51

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UniProt ID: (Human) P35226, (Mouse) P25916

View more View less

Entrez Gene ID: (Human) 648, (Mouse) 12151

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Function(s)

protein binding zinc ion binding RING-like zinc finger domain binding promoter-specific chromatin binding chromatin binding sequence-specific DNA binding metal ion binding ubiquitin-protein transferase activator activity

Process(es)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated segment specification regulation of gene expression covalent chromatin modification protein sumoylation hemopoiesis negative regulation of gene expression, epigenetic positive regulation of fibroblast proliferation positive regulation of ubiquitin-protein transferase activity skeletal system development in utero embryonic development DNA methylation regulation of transcription, DNA-templated humoral immune response brain development cellular process chromatin modification histone acetylation histone ubiquitination rostrocaudal neural tube patterning positive regulation of B cell proliferation positive regulation of immature T cell proliferation in thymus somatic stem cell division embryonic skeletal system morphogenesis embryonic skeletal system development regulation of catalytic activity hematopoietic stem cell homeostasis negative regulation of apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BMI1 Monoclonal Antibody (2E10A11)

BMI1 Antibody (66161-1-IG) in ICC/IF

Product Details

66161-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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