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|Tested species reactivity||Chem|
|Published species reactivity||Rat|
|Host / Isotype||Rat / IgG2a|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/25 - 1/200|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/25 - 1/200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-82718 detects free BrdU, BrdU attached to a protein carrier, and BrdU incorporated into single stranded DNA. This antibody does not cross-react with thymidine, but does weakly detect chlorodeoxyuridine.
For FACS analysis, use 20ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Basic fibroblast growth factor stimulates the proliferation and differentiation of neural stem cells in neonatal rats after ischemic brain injury.
MA1-82718 was used in immunocytochemistry to study neural stem cell proliferation and differentiation in neonatal rat brain following ischemic injury and the stimulatory effect of bFGF
|Jin-qiao S,Bin S,Wen-hao Z,Yi Y||Brain & development (31:331)||2009|
Hippocampal neurogenesis and gene expression depend on exercise intensity in juvenile rats.
MA1-82718 was used in immunohistochemistry to study the effects of exercise intensity on neurogenesis and gene expression in juvenile rat hippocampus
|Lou SJ,Liu JY,Chang H,Chen PJ||Brain research (1210:48)||2008|