Immunofluorescence analysis of CA-125 / MUC16 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CA-125 / MUC16 (Ov185:1) Mouse Monoclonal Antibody (Product # MA5-11579) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially purified mucin fraction from a pool of cancer tissue from patients with epithelial ovarian cancer|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11579 targets CA-125 in IHC (P) applications and shows reactivity with Human samples.
The MA5-11579 immunogen is partially purified mucin fraction from a pool of cancer tissue from patients with epithelial ovarian cancer.
As CA125 (Ovarian Cancer Antigen) is a tumour marker usually associated with ovarian epithelial malignancies. Serum assays are widely used to detect this protein in the monitoring of ovarian cancers. However, CA125 antigen may also be detected by immunohistochemistry and expression has been found in neoplasms such as seminal vesicle carcinoma and anaplastic lymphoma. CA125 antigen is not exclusively found in malignant tumours.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Dual use of a single Wilms' tumor 1 immunohistochemistry in evaluation of ovarian tumors: a preliminary study of 20 cases.
MA5-11579 was used in immunohistochemistry to study the use of Wilms' tumor 1 histochemistry to assess ovarian tumors
|Hsiao YH,Siddiqui S,Man YG||Journal of Cancer (1:93)||2010|
Prognostic significance of CA 125, CD44, and epithelial membrane antigen in renal cell carcinoma.
MA5-11579 was used in immunohistochemistry to study the prognostic value of CA 125, CD44 and epithelial membrane antigen in renal cell carcinoma
|Bamias A,Chorti M,Deliveliotis C,Trakas N,Skolarikos A,Protogerou B,Legaki S,Tsakalou G,Tamvakis N,Dimopoulos MA||Urology (62:368)||2003|
Multiple initial culture conditions enhance the establishment of cell lines from primary ovarian cancer specimens.
MA5-11579 was used in immunocytochemistry to establish stable ovarian tumor cell lines
|Bertozzi CC,Chang CY,Jairaj S,Shan X,Huang J,Weber BL,Chu CS,Carroll RG||In vitro cellular and developmental biology. Animal (42:58)||2006|