|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 223-268 of human CA5A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 35 kDa.
Purity is >95% by SDS-PAGE.
Carbonic anhydrases (CAs) are members of a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CAs are involved in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric juice. They show extensive diversity in distribution and in their subcellular localization. The human CA5A gene encodes for the CA V protein which is expressed in the mitochondrial matrix in liver tissues, but can also be detected in many other tissues. In ureagenesis, the CA V protein may be involved in providing bicarbonate to carbamoyl phosphate synthetase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.