Invitrogen

CCL5 (RANTES) Monoclonal Antibody (VL1), eFluor™ 660, eBioscience™

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CCL5 (RANTES) Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were cultured for 5 hours in the presence of monensin (Product # 00-4505-51). Cells were then stained with Anti-Human CD56 (NCAM) PE (Product # 12-0567-42) followed by fixation and permeabilization (Product # 88-8823-88) and subsequent intracellular staining with Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or Anti-Human CCL5 (RANTES) eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.

Product Details

50-9905-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b, kappa
Recommended Isotype Control	Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	VL1
Conjugate	eFluor™ 660
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574411

Product Specific Information

Description: The VL1 monoclonal antibody reacts with human CCL5/RANTES (Regulated upon Activation, Normal T cell Expressed and Secreted). RANTES is an 8 kDa member of the C-C chemokine family. Although rapidly induced in fibroblasts and epithelial cells, RANTES is upregulated only after 3-5 days of activation in T cells. As with other members of the C-C chemokine family, RANTES is a strong chemotractant for monocytes, NK cells, eosinophils and T cells. RANTES is also expressed by monocytes upon activation and has recently been reported to be constitutively expressed by NK cells.

Applications Reported: This VL1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This VL1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; RANTES(3-68); RANTES(4-68); regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

Gene Aliases: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228

UniProt ID: (Human) P13501

Entrez Gene ID: (Human) 6352

Molecular Function: cytokine intercellular signal molecule