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Invitrogen
| Applications | Tested Dilution | Publications |
|---|---|---|
Flow Cytometry (Flow) |
1:50-1:200 | - |
ELISA (ELISA) |
Assay-dependent | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Human / IgG4 |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
21E8 |
Immunogen |
Recombinant Human SEMA4D protein |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.4, with 50% glycerol |
Contains |
0.03% Proclin 300 |
Storage conditions |
-20°C or -80°C if preferred |
Antibody tested in Indirect ELISA
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A8; BB18; CD100; GR3; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; Semaphorin-4D
Gene Aliases: C9orf164; CD100; coll-4; M-sema-G; SEMA4D; SEMAJ
UniProt ID: (Human) Q92854
Entrez Gene ID: (Human) 10507
Molecular Function:
membrane-bound signaling molecule
intercellular signal molecule
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