Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), eBioscience™

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CD106 (VCAM-1) Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 3 Loss of endothelial SENP1 inhibits EC activation. ( a ) Grafts from WT or SENP1-ecKO mice were harvested 3 days post-transplantation. The induction of endothelial adhesion molecules was demonstrated by immunofluorescence staining of ICAM-1, VCAM-1, or P-selectin and PECAM-1 with DAPI labelling of the nuclei. Bar represents 50 mum. ( b - e ) Attenuated induction of adhesion molecules in SENP1-ecKO MAECs. Flow cytometry analysis of ICAM-1, VCAM-1 and P-selectin in MAECs isolated from WT or SENP1-ecKO mice after TNF or IL-1beta treatment. Representative histograms are shown in ( b ) with the quantification of mean intensity in ( c - e ). ( f - h ) Overexpression of the catalytically inactive form of SENP1 (SENP1-Mut) inhibits the induction of adhesion molecules in HUVECs. HUVECs were infected by Ad-SENP1-Mut or vector control (Ad-LacZ) for 24 h, treated with pro-inflammatory cytokines and analysed by flow cytometry in the same way as MAECs. Representative histograms of ICAM-1 and VCAM-1 are shown in ( f ) with the quantification of mean intensity in ( g , h ). Data are presented as the mean+-s.e.m. from at least three independent experiments. * P <0.05 and ** P <0.01; two-way ANOVA followed by Bonferroni post-test. MAEC, mouse aortic endothelial cell.

CD106 (VCAM-1) Antibody in Immunohistochemistry (IHC) — Figure 6 Changes in aortic root and aortic arch mRNA expression for cytokines and adhesion molecules (tumor necrosis factor alpha, chemokine (C-C motif) ligand 2, intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) determined using RT-PCR in wild-type mice. (A) Relative expression at days 1, 3 and 5 days are presented. CCL2, Chemokine (C-C motif) ligand 2; CLP, Cecal ligation and puncture; TNF-alpha, Tumor necrosis factor alpha. Immunofluorescent images of the aortic root obtained to determine the expression of adhesion molecules intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) (left to right: secondary antibody-only control, sham, cecal ligation and puncture (CLP)). ICAM-1 is shown in green, VCAM-1 in red and nuclear material in blue (4',6-diamidino-2-phenylindole dihydrochloride (DAPI)). Inset shows possible colocalization of ICAM-1 with VCAM-1. (B) Graph presents cell adhesion to inflamed aortic endothelium at days 1, 3 and 5 days. Representative images show monocyte adhesion to the endothelium by day 5 (* P < 0.05 compared to the time-matched sham-treated groups).

CD106 (VCAM-1) Antibody in Flow Cytometry (Flow) — Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Product Details

14-1061-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.25 µg/test	View 9 publications 9 publications
Immunoprecipitation (IP)	Assay-Dependent	-
Neutralization (Neu)	Assay-Dependent	View 1 publication 1 publication
Functional Assay (FN)	Assay-Dependent	-

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Rat / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	429
Conjugate	Unconjugated APC Biotin eFluor 450 eFluor 660 FITC Functional Grade PE PE-Cyanine7
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_467419

Product Specific Information

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections (IHC-F). It has also been reported in blocking of CD106 in functional studies. (Please use Functional Grade purified 429, cat. 16-1061, in functional assays.).

Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Defining the role of pulmonary endothelial cell heterogeneity in the response to acute lung injury.

eLife

Niethamer TK,Stabler CT,Leach JP,Zepp JA,Morley MP,Babu A,Zhou S,Morrisey EE

14-1061 was used in Immunohistochemistry to identify that different subtypes of endothelial cells exist in capillaries, and that some may be able to perform slightly different jobs during lung recovery.

Mon Feb 24 00:00:00 EST 2020

Regulatory T Cells Condition Lymphatic Endothelia for Enhanced Transendothelial Migration.

Cell reports

Piao W,Xiong Y,Li L,Saxena V,Smith KD,Hippen KL,Paluskievicz C,Willsonshirkey M,Blazar BR,Abdi R,Bromberg JS

14-1061 was used in Immunohistochemistry-immunofluorescence to demonstrate the role of regulatory T cells in enhancing leukocyte migration through endothelial barriers.

Tue Jan 28 00:00:00 EST 2020

Leptin Induces Proadipogenic and Proinflammatory Signaling in Adipocytes.

Frontiers in endocrinology

Palhinha L,Liechocki S,Hottz ED,Pereira JADS,de Almeida CJ,Moraes-Vieira PMM,Bozza PT,Maya-Monteiro CM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Mon Jan 13 00:00:00 EST 2020

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

Inhibition of the JNK/MAPK signaling pathway by myogenesis-associated miRNAs is required for skeletal muscle development.

Cell death and differentiation

Xie SJ,Li JH,Chen HF,Tan YY,Liu SR,Zhang Y,Xu H,Yang JH,Liu S,Zheng LL,Huang MB,Guo YH,Zhang Q,Zhou H,Qu LH

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Polymicrobial sepsis impairs bystander recruitment of effector cells to infected skin despite optimal sensing and alarming function of skin resident memory CD8 T cells.

PLoS pathogens

Danahy DB,Anthony SM,Jensen IJ,Hartwig SM,Shan Q,Xue HH,Harty JT,Griffith TS,Badovinac VP

14-1061-82 was used in Flow Cytometry to directly examine the impact of sepsis on tissue resident memory CD8+ T cells in mice.

Fri Sep 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

A robust in vitro model for trans-lymphatic endothelial migration.

Scientific reports

Xiong Y,Brinkman CC,Famulski KS,Mongodin EF,Lord CJ,Hippen KL,Blazar BR,Bromberg JS

14-1061 was used in Flow cytometry/Cell sorting to establish an in vitro system to evaluate lymphatic Trans-endothelial migration for various cell types across primary mouse and human lymphatic endothelial cells.

Tue May 09 00:00:00 EDT 2017

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Tue Jun 21 00:00:00 EDT 2016

Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.

Nature communications

Peske JD,Thompson ED,Gemta L,Baylis RA,Fu YX,Engelhard VH

14106182 was used in flow cytometry and immunohistochemistry to find that lymph node-like vasculature in melanoma and lung carcinoma murine models is both a consequence of and key contributor to anti-tumor immunity

Wed May 13 00:00:00 EDT 2015

IgE-activated basophils regulate eosinophil tissue entry by modulating endothelial function.

The Journal of experimental medicine

Cheng LE,Sullivan BM,Retana LE,Allen CD,Liang HE,Locksley RM

14-1061 was used in Flow cytometry/Cell sorting to study the role of basophils in IgE-dependent eosinophilic dermatitis.

Mon Apr 06 00:00:00 EDT 2015

Neutrophil crawling in capillaries; a novel immune response to Staphylococcus aureus.

PLoS pathogens

Harding MG,Zhang K,Conly J,Kubes P

14-1061 was used in Blocking experiments to elucidate the importance of neutrophils in protection against MRSA-induced soft tissue infections.

Wed Oct 01 00:00:00 EDT 2014

Effects of intra-abdominal sepsis on atherosclerosis in mice.

Critical care (London, England)

Kaynar AM,Yende S,Zhu L,Frederick DR,Chambers R,Burton CL,Carter M,Stolz DB,Agostini B,Gregory AD,Nagarajan S,Shapiro SD,Angus DC

Wed Sep 03 00:00:00 EDT 2014

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD106 (VCAM-1) monoclonal antibody (Product # 14-1061-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Influence of fatty acids in maternal diet on atherogenesis in offspring of LDL receptor-deficient mice.

International journal of clinical and experimental medicine

de Oliveira Cipriano Torres D,Santos AC,Silva AK,de Moura PM,Beltrão EI,Peixoto CA

14-1061 was used in Immunohistochemistry on paraffin embedded tissues to demonstrate that hypercholesterolemic mothers fed a diet rich in omega-6 may predispose their offspring to endothelial dysfunction.