Invitrogen

CD106 (VCAM-1) Monoclonal Antibody (429), eBioscience™

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CD106 (VCAM-1) Antibody — Figure 1 Low doses of ionizing radiation have no significant impact on cell death and migration properties of FLS cultures of healthy mice. Healthy FLS cultures obtained from C57Bl/6 mice were phenotyped at passage 5, seeded and analyzed 96 h after the treatment with low, intermediate, and high doses of ionizing radiation. Cell pools that were tested via flow cytometry were considered to be FLS when being CD11b - ( A.1 ), CD54 + ( A.2 ), and CD106 + ( A.3 ). B.1 to B.3 shows gating strategy for cell death analysis of FLS via flow cytometry. Cell death was determined after staining of the cells ( B.1 ., total scatter) with AxV-FITC/PI ( B.2 , B.3 ). Vital cells were defined as AxV - /PI - , necrotic ones as PI + ( B.2 , E ), and apoptotic cells as AxV + , but PI - ( B.3 , D ). Cell numbers ( C ) of living FLS were counted using a Neubauer chamber. Cell migration and invasiveness was tested via scratch assay ( F ) and matrigel invasion assay ( G ). For the scratch assay, cells were irradiated after a scratch was applied; pictures were taken every 24 h and scratch area was quantified using Tscratch (V1.0, Copyright T.Geback, M.Schulz, Zurich, Switzerland). The initial scratch area was set to 100% and scratch area of the consecutive days was calculated accordingly. For assessment of invasiveness cells were seeded in a matrigel coated transwell, irradiated and incubated for 3 days. Cells were then stained with crystal violet and invaded area was assessed using Image

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.25 µg/test

Functional Assay (FN)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

Published Applications

Flow Cytometry (Flow)

See 5 publications below

Immunofluorescence (IF)

See 2 publications below

Immunohistochemistry (IHC)

See 1 publication below

Neutralization (Neu)

See 1 publication below

Immunohistochemistry (Paraffin) (IHC (P))

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

429

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_467419

Product Specific Information

Description: The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and Integrin a4b7 (LPAM-1) and these interactions in the bone marrow and thymus are important for early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: This 429 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections (IHC-F). It has also been reported in blocking of CD106 in functional studies. (Please use Functional Grade purified 429, cat. 16-1061, in functional assays.).

Applications Tested: The 429 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.