Invitrogen

CD11c Monoclonal Antibody (N418), eFluor™ 615, eBioscience™

Product Image Gallery

CD11c Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry on frozen mouse spleen using 5 µg/mL Anti-Mouse CD11c eFluor® 615. Nuclei are counterstained with DAPI.

CD11c Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunocytochemistry

CD11c Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F)) — Figure 1. IL-15 -/- mice fail to develop P. acnes - and zymosan-induced granulomas. (A) Granuloma formation in the liver of WT, IL-15 -/- , RAG-2 -/- , and IL-15 -/- RAG-2 -/- mice. Livers were taken on day 6 after a 0.5-mg heat-killed P. acnes injection, and sections were stained with H&E. Bar, 100 mum. (B) The liver sections of P. acnes -injected WT and IL-15 -/- mice were stained with biotinylated anti-CD11c mAb and streptavidin-HRP and further visualized with DAB. Slides were counterstained with Mayer's hematoxylin. Bar, 100 mum. (C) Chemokine levels in the sera of WT, IL-15 -/- , RAG-2 -/- , and IL-15 -/- RAG-2 -/- mice were assessed by ELISA at 72 h after a 0.5-mg heat-killed P. acnes injection. Values represent SD ( n = 3 mice/group). Data are representative of two to four experiments. (D) Granuloma formation in the liver of zymosan-injected WT and IL-15 -/- mice. Livers were taken on day 6 after a 1 mg zymosan injection, and sections were stained with biotinylated anti-CD11c mAb and streptavidin-HRP and further visualized with DAB. Slides were counterstained with Mayer's hematoxylin. Bar, 100 mum. (E) The liver sections of P. acnes -injected WT and IFN-gamma -/- mice were stained as described in B. Bar, 100 mum. (F) 3 d after a 1-mg zymosan injection, CCL2 in the sera of WT and IL-15 -/- mice was measured by ELISA. Data are representative of four experiments. (G) 3 d after a 0.5-mg P. acnes injection, IL-12p70, and IFN-gamma in the sera of WT and IL-15 -/- m

CD11c Antibody in Immunohistochemistry (IHC) — Figure 6 Glufosinate treatment promotes M1-like TAMs, CTL tumor accumulation, vessel pruning, and normalization and inhibits metastasis in a murine model of melanoma A Experimental plan. Mice were intradermally injected with YUMM 1.7 (CD90.1 + ) cells. When the tumor reached 80-100 mm 3 size, mice were treated by gavage with glufosinate (20 mg/kg) or vehicle for 14 days. B, C Intradermal YUMM 1.7 (CD90.1 + ) tumor growth over the time (B) and number of metastatic lesions per lung (C) in vehicle and glufosinate (20 mg/kg)-treated mice (pool of 2 independent experiments; 10 mice per condition in total). D Representative images of metastatic lesions checked by DAB-H stain for CD90.1 positivity in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). Six images per lung were analyzed. Scale bar: 2 mm. Zoomed area scale bar: 50 mum. E, F FACS quantification of M1-like MHC class II high (E) and MHC class II low (F) CD11c + CD206 - TAMs over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). G Quantification and representative images of CD11c + cells over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). Six images per tumor were analyzed. Scale bars: 50 mum. H, I FACS quantification of M2-like MHC class II high (H) and MHC class II low (I) CD11c - CD206 + TAMs over the total number of F4/80 + cells in vehicle and glufosinate (20 mg/kg)-treated mice ( n = 6). J Quantification and representat

Product Details

42-0114-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 13 publications 13 publications
Immunohistochemistry (Frozen) (IHC (F))	5 µg/mL	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	Assay-Dependent	View 5 publications 5 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Human, Mouse
Host/Isotype	Armenian hamster / IgG
Recommended Isotype Control	Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 615, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	N418
Conjugate	eFluor™ 615 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 660 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 eFluor 506 eFluor 660 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 610-30S NovaFluor Blue 660-40S NovaFluor Yellow 570 PE PE-Cyanine5 PE-Cyanine5.5 PE-Cyanine7 PE-eFluor 610 PerCP-Cyanine5.5 Super Bright 436
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10853016

Product Specific Information

Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported: This N418 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunocytochemistry.

Applications Tested: This N418 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Wed Oct 07 00:00:00 EDT 2020

Endothelial-to-mesenchymal transition compromises vascular integrity to induce Myc-mediated metabolic reprogramming in kidney fibrosis.

Science signaling

Lovisa S,Fletcher-Sananikone E,Sugimoto H,Hensel J,Lahiri S,Hertig A,Taduri G,Lawson E,Dewar R,Revuelta I,Kato N,Wu CJ,Bassett RL,Putluri N,Zeisberg M,Zeisberg EM,LeBleu VS,Kalluri R

42-0114 was used in Flow cytometry/Cell sorting to demonstrate a functional role for EndMT in the response to kidney capillary endothelial injury and highlight the contribution of endothelial-epithelial cross-talk in the development of kidney fibrosis with a potential for therapeutic intervention.

Tue Jun 09 00:00:00 EDT 2020

Identification of different macrophage subpopulations with distinct activities in a mouse model of oxygen-induced retinopathy.

International journal of molecular medicine

Zhu Y,Zhang L,Lu Q,Gao Y,Cai Y,Sui A,Su T,Shen X,Xie B

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2017

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Wed Apr 12 00:00:00 EDT 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Wed Apr 05 00:00:00 EDT 2017

PEDF mediates pathological neovascularization by regulating macrophage recruitment and polarization in the mouse model of oxygen-induced retinopathy.

Scientific reports

Gao S,Li C,Zhu Y,Wang Y,Sui A,Zhong Y,Xie B,Shen X

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Fri Feb 17 00:00:00 EST 2017

Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis.

Biochimica et biophysica acta

Hersrud SL,Kovács AD,Pearce DA

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2016

Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor.

Journal of cellular physiology

Li Y,Adomat H,Guns ET,Hojabrpour P,Duronio V,Curran TA,Jalili RB,Jia W,Delwar Z,Zhang Y,Elizei SS,Ghahary A

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunocytochemistry

Wed Jun 01 00:00:00 EDT 2016

Flagellin is a strong vaginal adjuvant of a therapeutic vaccine for genital cancer.

Oncoimmunology

Lee SE,Hong SH,Verma V,Lee YS,Duong TN,Jeong K,Uthaman S,Sung YC,Lee JT,Park IK,Min JJ,Rhee JH

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Mon Feb 01 00:00:00 EST 2016

M cell-derived vesicles suggest a unique pathway for trans-epithelial antigen delivery.

Tissue barriers

Sakhon OS,Ross B,Gusti V,Pham AJ,Vu K,Lo DD

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Fri Apr 03 00:00:00 EDT 2015

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Mon Dec 01 00:00:00 EST 2014

Invariant natural killer T cells regulate anti-tumor immunity by controlling the population of dendritic cells in tumor and draining lymph nodes.

Journal for immunotherapy of cancer

Pilones KA,Aryankalayil J,Babb JS,Demaria S

42-0114 was used in Immunohistochemistry to demonstrate that iNKT cells limit priming of anti-tumor T-cells by controlling dendritic cells in tumors and draining lymph nodes.

Tue Oct 28 00:00:00 EDT 2014

Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans.

PloS one

Itoh M,Kato H,Suganami T,Konuma K,Marumoto Y,Terai S,Sakugawa H,Kanai S,Hamaguchi M,Fukaishi T,Aoe S,Akiyoshi K,Komohara Y,Takeya M,Sakaida I,Ogawa Y

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Sun Oct 12 00:00:00 EDT 2014

Mice deficient in intestinal epithelium cytochrome P450 reductase are prone to acute toxin-induced mucosal damage.

Scientific reports

Ahlawat S,Xie F,Zhu Y,D'Hondt R,Ding X,Zhang QY,Mantis NJ

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Thu Jul 03 00:00:00 EDT 2014

Sessile alveolar macrophages communicate with alveolar epithelium to modulate immunity.

Nature

Westphalen K,Gusarova GA,Islam MN,Subramanian M,Cohen TS,Prince AS,Bhattacharya J

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Thu Feb 27 00:00:00 EST 2014

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Miscellaneous

Tue Jan 01 00:00:00 EST 2013

Depletion of B2 but not B1a B cells in BAFF receptor-deficient ApoE mice attenuates atherosclerosis by potently ameliorating arterial inflammation.

PloS one

Kyaw T,Tay C,Hosseini H,Kanellakis P,Gadowski T,MacKay F,Tipping P,Bobik A,Toh BH

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Mon May 14 00:00:00 EDT 2012

Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.

Journal of neurochemistry

Koronyo-Hamaoui M,Ko MK,Koronyo Y,Azoulay D,Seksenyan A,Kunis G,Pham M,Bakhsheshian J,Rogeri P,Black KL,Farkas DL,Schwartz M

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Tue Dec 01 00:00:00 EST 2009

Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.

The Journal of endocrinology

Gruver AL,Ventevogel MS,Sempowski GD

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2009

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Mon Dec 01 00:00:00 EST 2008

Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo.

The Journal of experimental medicine

Ohteki T,Tada H,Ishida K,Sato T,Maki C,Yamada T,Hamuro J,Koyasu S

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Mon Oct 02 00:00:00 EDT 2006

Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo.

The Journal of experimental medicine

Ohteki T,Tada H,Ishida K,Sato T,Maki C,Yamada T,Hamuro J,Koyasu S

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Mon Oct 02 00:00:00 EDT 2006

The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties.

International immunology

Zhang J,Kawashima N,Suda H,Nakano Y,Takano Y,Azuma M

Fri Sep 01 00:00:00 EDT 2006

Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.

Cancer research

Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP

Fri Apr 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement receptor 4; Integrin alpha-X; integrin aX; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95

Gene Aliases: AI449405; Cd11c; Cr4; Itgax; N418

UniProt ID: (Mouse) Q9QXH4

Entrez Gene ID: (Mouse) 16411

Molecular Function: integrin