Invitrogen

CD127 Monoclonal Antibody (A7R34), Alexa Fluor™ 488, eBioscience™

Product Image Gallery

CD127 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.5 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.5 µg of Anti-Mouse CD127 Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.

Product Details

53-1271-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A7R34

Conjugate

Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_469909

Product Specific Information

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Sep 08 00:00:00 EDT 2021

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

Zhang Z,Huang Z,Ong B,Sahu C,Zeng H,Ruan HB

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Apr 16 00:00:00 EDT 2019

Activation of naïve CD4⁺ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4⁺ T cells.

Nature immunology

Twohig JP,Cardus Figueras A,Andrews R,Wiede F,Cossins BC,Derrac Soria A,Lewis MJ,Townsend MJ,Millrine D,Li J,Hill DG,Uceda Fernandez J,Liu X,Szomolay B,Pepper CJ,Taylor PR,Pitzalis C,Tiganis T,Williams NM,Jones GW,Jones SA

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Mon Apr 01 00:00:00 EDT 2019

The interplay of BMP4 and IL‑7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia̸reperfusion.

International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

The interplay of BMP4 and IL‑7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia̸reperfusion.

International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Mar 28 00:00:00 EDT 2018

Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.

mBio

Douam F,Soto Albrecht YE,Hrebikova G,Sadimin E,Davidson C,Kotenko SV,Ploss A

53-1271 was used in Flow cytometry/Cell sorting to analyse the role of type III interferon (IFN)-mediated signaling in controlling the infection and attenuation of the live attenuated yellow virus strain, YFV-17D.

Tue Aug 15 00:00:00 EDT 2017

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

53-1271 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 EDT 2017

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Mar 09 00:00:00 EST 2016

Partial Loss of Rpl11 in Adult Mice Recapitulates Diamond-Blackfan Anemia and Promotes Lymphomagenesis.

Cell reports

Morgado-Palacin L,Varetti G,Llanos S,Gómez-López G,Martinez D,Serrano M

53-1271 was used in Flow cytometry/Cell sorting to conclude that Rpl11-deficient mice recapitulate DBA disorder, including cancer predisposition.

Tue Oct 27 00:00:00 EDT 2015

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation.

Nature immunology

Ochiai K,Maienschein-Cline M,Mandal M,Triggs JR,Bertolino E,Sciammas R,Dinner AR,Clark MR,Singh H

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sun Jan 22 00:00:00 EST 2012

Common gamma-chain-dependent signals confer selective survival of eosinophils in the murine small intestine.

Journal of immunology (Baltimore, Md. : 1950)

Carlens J,Wahl B,Ballmaier M,Bulfone-Paus S,Förster R,Pabst O

53-1271 was used in Flow cytometry/Cell sorting to analyse the phenotype of eosinophils in the intestines of mice.

Sun Nov 01 00:00:00 EDT 2009

LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.

Journal of immunology (Baltimore, Md. : 1950)

Vondenhoff MF,Greuter M,Goverse G,Elewaut D,Dewint P,Ware CF,Hoorweg K,Kraal G,Mebius RE

Fri May 01 00:00:00 EDT 2009

Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.

The American journal of pathology

Leithäuser F,Meinhardt-Krajina T,Fink K,Wotschke B,Möller P,Reimann J

Thu Jun 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: CD127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

Gene Aliases: CD127; IL-7Ralpha; Il7r

UniProt ID: (Mouse) P16872

Entrez Gene ID: (Mouse) 16197

Molecular Function: transmembrane signal receptor

Function(s)

cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus B cell proliferation lymph node development homeostasis of number of cells

Process(es)

cytokine receptor activity

CD127 Monoclonal Antibody (A7R34), Alexa Fluor™ 488, eBioscience™

Product Details

53-1271-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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