Invitrogen

CD127 Monoclonal Antibody (A7R34), Alexa Fluor™ 488, eBioscience™

23 Published Figures

27 References

View all (126) CD127 antibodies

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (23)

FIGURE: 1 / 24

CD127 Antibody (53-1271-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.5 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.5 µg of Anti-Mouse CD127 Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.

CD127 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-1271-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 22 publications 22 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A7R34

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_469909

Product Specific Information

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127, also known as the Interleukin-7 receptor alpha chain (IL-7Ralpha), is a type I glycoprotein with a molecular weight of 75-80 kDa. It forms a multi-functional receptor complex with CD132, the common gamma chain, to create the IL-7 receptor (IL-7R), which is crucial for lymphopoiesis. The active IL-7 receptor is an alpha/gamma chain heterodimer, where the gamma chain also associates with the interleukin-2 receptor and primarily activates signal transduction, while the alpha chain determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 is expressed by immature B cells through the early pre-B stage, thymocytes during various stages of development, and most mature T cells, with transient down-regulation upon activation. It promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. Binding of IL-7 to CD127 results in signal transduction through several tyrosine kinase pathways, including the Jak/STAT pathway, and is indispensable for lymphocyte development and the control of homeostatic proliferation of T cells in the periphery. Additionally, IL-7R signaling is involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells. Interestingly, CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While CD4 and CD25 co-expression is commonly used to identify T regs, this method may include cells without suppressive activity. CD4+CD25+ cells that have down-regulated CD127 expression are significantly enriched for the regulatory T cell population, as defined by the expression of the T reg-specific transcription factor Foxp3 and their suppressive activity in vitro. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

New Insights into Roles of IL-7R Gene as a Therapeutic Target Following Intracerebral Hemorrhage.

Journal of inflammation research

Cui J,Wang H,Liu S,Zhao Y

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Western Blot

Wed Oct 23 00:00:00 UTC 2024

An essential role for miR-15/16 in Treg suppression and restriction of proliferation.

Cell reports

Johansson K,Gagnon JD,Zhou SK,Fassett MS,Schroeder AW,Kageyama R,Bautista RA,Pham H,Woodruff PG,Ansel KM

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Oct 31 00:00:00 UTC 2023

Role of Nox4 in Mitigating Inflammation and Fibrosis in Dextran Sulfate Sodium-Induced Colitis.

Cellular and molecular gastroenterology and hepatology

Lee Y,Kim SH,Jeong H,Kim KH,Jeon D,Cho Y,Lee D,Nam KT

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Immunohistochemistry

Tue Aug 22 00:00:00 UTC 2023

c-Myc uses Cul4b to preserve genome integrity and promote antiviral CD8⁺ T cell immunity.

Nature communications

Dar AA,Kim DD,Gordon SM,Klinzing K,Rosen S,Guha I,Porter N,Ortega Y,Forsyth KS,Roof J,Fazelinia H,Spruce LA,Eisenlohr LC,Behrens EM,Oliver PM

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sat Nov 04 00:00:00 UTC 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2023

MicroRNA‑21‑5p protects melanocytes via targeting STAT3 and modulating Treg/Teff balance to alleviate vitiligo.

Molecular medicine reports

Huo J,Liu T,Li F,Song X,Hou X

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Oct 12 00:00:00 UTC 2021

Hedgehog interacting protein-expressing lung fibroblasts suppress lymphocytic inflammation in mice.

JCI insight

Yun JH,Lee C,Liu T,Liu S,Kim EY,Xu S,Curtis JL,Pinello L,Bowler RP,Silverman EK,Hersh CP,Zhou X

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Sep 08 00:00:00 UTC 2021

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Fri Mar 26 00:00:00 UTC 2021

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Apr 16 00:00:00 UTC 2019

Activation of naïve CD4⁺ T cells re-tunes STAT1 signaling to deliver unique cytokine responses in memory CD4⁺ T cells.

Nature immunology

Twohig JP,Cardus Figueras A,Andrews R,Wiede F,Cossins BC,Derrac Soria A,Lewis MJ,Townsend MJ,Millrine D,Li J,Hill DG,Uceda Fernandez J,Liu X,Szomolay B,Pepper CJ,Taylor PR,Pitzalis C,Tiganis T,Williams NM,Jones GW,Jones SA

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Mon Apr 01 00:00:00 UTC 2019

Bone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis.

Haematologica

Zhang Z,Huang Z,Ong B,Sahu C,Zeng H,Ruan HB

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sun Sep 01 00:00:00 UTC 2019

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue Dec 03 00:00:00 UTC 2019

Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Wang Y,Xiao M,Tao C,Chen J,Wang Z,Yang J,Chen Z,Zou Z,Liu A,Cai D,Jiang Y,Ding C,Li M,Bai X

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sun Apr 01 00:00:00 UTC 2018

The interplay of BMP4 and IL‑7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia̸reperfusion.

International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue May 01 00:00:00 UTC 2018

The interplay of BMP4 and IL‑7 regulates the apoptosis of intestinal intraepithelial lymphocytes under conditions of ischemia̸reperfusion.

International journal of molecular medicine

Luo B,Chen K,Feng Q,Xiao W,Ma D,Yang H,Zhang C

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Tue May 01 00:00:00 UTC 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Mar 28 00:00:00 UTC 2018

Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome.

Nature communications

Douam F,Hrebikova G,Albrecht YE,Sellau J,Sharon Y,Ding Q,Ploss A

53-1271 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models.

Tue Mar 14 00:00:00 UTC 2017

Type III Interferon-Mediated Signaling Is Critical for Controlling Live Attenuated Yellow Fever Virus Infection In Vivo.

mBio

Douam F,Soto Albrecht YE,Hrebikova G,Sadimin E,Davidson C,Kotenko SV,Ploss A

53-1271 was used in Flow cytometry/Cell sorting to analyse the role of type III interferon (IFN)-mediated signaling in controlling the infection and attenuation of the live attenuated yellow virus strain, YFV-17D.

Tue Aug 15 00:00:00 UTC 2017

A simple, accurate and universal method for quantification of PCR.

BMC biotechnology

Boulter N,Suarez FG,Schibeci S,Sunderland T,Tolhurst O,Hunter T,Hodge G,Handelsman D,Simanainen U,Hendriks E,Duggan K

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Wed Mar 09 00:00:00 UTC 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Partial Loss of Rpl11 in Adult Mice Recapitulates Diamond-Blackfan Anemia and Promotes Lymphomagenesis.

Cell reports

Morgado-Palacin L,Varetti G,Llanos S,Gómez-López G,Martinez D,Serrano M

53-1271 was used in Flow cytometry/Cell sorting to conclude that Rpl11-deficient mice recapitulate DBA disorder, including cancer predisposition.

Tue Oct 27 00:00:00 UTC 2015

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Mon Sep 22 00:00:00 UTC 2014

A self-reinforcing regulatory network triggered by limiting IL-7 activates pre-BCR signaling and differentiation.

Nature immunology

Ochiai K,Maienschein-Cline M,Mandal M,Triggs JR,Bertolino E,Sciammas R,Dinner AR,Clark MR,Singh H

Published figure using CD127 monoclonal antibody (Product # 53-1271-82) in Flow Cytometry

Sun Jan 22 00:00:00 UTC 2012

LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.

Journal of immunology (Baltimore, Md. : 1950)

Vondenhoff MF,Greuter M,Goverse G,Elewaut D,Dewint P,Ware CF,Hoorweg K,Kraal G,Mebius RE

Fri May 01 00:00:00 UTC 2009

Common gamma-chain-dependent signals confer selective survival of eosinophils in the murine small intestine.

Journal of immunology (Baltimore, Md. : 1950)

Carlens J,Wahl B,Ballmaier M,Bulfone-Paus S,Förster R,Pabst O

53-1271 was used in Flow cytometry/Cell sorting to analyse the phenotype of eosinophils in the intestines of mice.

Sun Nov 01 00:00:00 UTC 2009

Foxp3-expressing CD103+ regulatory T cells accumulate in dendritic cell aggregates of the colonic mucosa in murine transfer colitis.

The American journal of pathology

Leithäuser F,Meinhardt-Krajina T,Fink K,Wotschke B,Möller P,Reimann J

Thu Jun 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: CD127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

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Gene Aliases: CD127; IL-7Ralpha; Il7r

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UniProt ID: (Mouse) P16872

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Entrez Gene ID: (Mouse) 16197

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Function(s)

cytokine receptor activity transmembrane signal receptor

Process(es)

cell morphogenesis negative regulation of T cell mediated cytotoxicity immunoglobulin production regulation of cell size positive regulation of gene expression cell growth T cell differentiation positive regulation of T cell differentiation in thymus B cell proliferation lymph node development homeostasis of number of cells

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD127 Monoclonal Antibody (A7R34), Alexa Fluor™ 488, eBioscience™

CD127 Antibody (53-1271-82) in Flow

Product Details

53-1271-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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