Invitrogen

CD127 Monoclonal Antibody (A7R34), Alexa Fluor 488, eBioscience™

Product Image Gallery

CD127 Antibody — Published figure using CD127 monoclonal antibody (Product # 53-1271-82)

CD127 Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.5 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.5 µg of Anti-Mouse CD127 Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1 µg/test

Published Applications

Flow Cytometry (Flow)

See 9 publications below

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host / Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A7R34

Conjugate

Alexa Fluor® 488

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_469909

Product Specific Information

Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Applications Reported: This A7R34 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD127; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; IL-7Ralpha; interleukin 7 receptor alpha chain; Interleukin-7 receptor subunit alpha; MGC107557

Gene Aliases: CD127; IL-7Ralpha; Il7r

UniProt ID: (Mouse) P16872

Entrez Gene ID: (Mouse) 16197

Molecular Function: cytokine receptor receptor type I cytokine receptor