Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.
Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha
Gene Aliases: AI115593; CD140a; Pdgfr-2; Pdgfra
UniProt ID: (Mouse) P26618
Entrez Gene ID: (Mouse) 18595