Invitrogen

CD140a (PDGFRA) Monoclonal Antibody (APA5), PE-eFluor™ 610, eBioscience™

Product Image Gallery

CD140a (PDGFRA) Antibody in Flow Cytometry (Flow) — NIH/3T3 cells were stained with 0.125 µg of Rat IgG2a kappa Isotype Control, PE-eFluor 610 (Product # 61-4321-82) (blue histogram) or 0.125 µg of CD140a Monoclonal Antibody, PE-eFluor 610 (purple histogram). Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).

CD140a (PDGFRA) Antibody in Immunocytochemistry (ICC/IF) — FIGURE 2 PSyC is mediated by EVs. (a) Schematic diagram of the chimeric aggregate co-culture differentiation system with EV secretion inhibited. PKA-ESCs and Control-ESCs were seeded in low adhesion dishes at a 3:1 ratio to create chimeric aggregates, and differentiation induction was initiated by the depletion of LIF. 24 h later, the aggregates were replated on normal plates and cultured with EV secretion inhibitors. (b) The number of Flk1 + cells was reduced with treatment of the EV secretion inhibitors manumycin A (10 muM) or GW4869 (5 muM). PKA-ESC and Control-ESC chimeric aggregates on D3.5 under Dox- condition were analysed by FACS. (c) Percentage of PKA-ESCs and Control-ESCs in chimeric aggregates expressing Flk1 under Dox- condition. Data were analysed using the Kruskal-Wallis test followed by the Steel-Dwass test. (d) Immunostaining for Flk1 + Control-ESCs (white) in chimeric aggregates with manumycin A (10 muM), GW4869 (5 muM), or DMSO (control). (e) Schematic diagram of the EV collection and treatment. Control-ESCs aggregates were plated without LIF. EVs were collected from the conditioned medium of PKA-ESCs as pellets and added to Control-ESCs aggregates. (f) Immunoblots of EV markers CD81 and Flotillin-1, non-EV marker calnexin in the cell or EV lysate (30 mug for CD81 and calnexin, 3 mug for Flotillin-1). (g) Transmission electron microscopy image of EVs from the conditioned medium. (h) Analysis of the EV size distribution in Dox+ (left) and Dox- (right) conditi

CD140a (PDGFRA) Antibody in Immunohistochemistry (IHC) — Extended Data Fig. 6 Oligodendrocyte lineage progression during the postnatal period. a , Immunofluorescent staining of Olig2 and CC1 in inter-lobule VI-VII WM at P30. Scale bar, 60 mum. b , Quantification of Olig2+ and CC1 + cell density in the male cerebellar WM at P30. Data presented as means +- SEM. Two-way ANOVA with Sidak's multiple comparisons test (***p < 0.005, ****p < 0.0001). Olig2: n = 7 C and 8 plKO (p < 0.0001); CC1: n = 6 C and 7plKO (p = 0.0005). c , Quantification of BrdU + /Olig2+ cell density in the male cerebellar WM at P30. BrdU (50 mg/kg) was injected to dams at E15.5. Data presented as means +- SEM. Two-tailed unpaired Student's t test with Welch's correction (*p < 0.05). n = 5 C and 5plKO (p = 0.0107). d , Quantification of Olig2+ cell density in the male cerebellar WM at P7 and P15. Data is presented as means +- SEM. Two-way ANOVA with Sidak's multiple comparisons test (***p < 0.005, ****p < 0.00010). P7: n = 3 mice/group (p = 0.0026); P15: n = 6 mice/group (p < 0.0001). The density of Olig2+ cells in the cerebellar WM is significantly increased in plKO males as compared with C littermates. e , OPC (PDGFRalpha + cell) density at P7, P15 and P30. Data is presented as means +- SEM. Two-way ANOVA with Sidak's multiple comparisons test (*p < 0.05). P7: n = 3 mice/group (p = 0.0435); P15: n = 6 mice/group (p = 0.3644); P30: n = 6 C and 8 plKO (p = 0.8558). The density of OPCs in the cerebellar WM is transiently higher in plKO males as compared with C litte

Product Details

61-1401-82

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	0.25 µg/test	View 18 publications 18 publications

Product Specifications
Species Reactivity	Mouse
Published species	Not Applicable
Host/Isotype	Rat / IgG2a, kappa
Recommended Isotype Control	Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor™ 610, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	APA5
Conjugate	PE-eFluor™ 610 APC APC-eFluor 780 Biotin FITC Functional Grade PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 702 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2811919

Product Specific Information

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

An estrogen-sensitive fibroblast population drives abdominal muscle fibrosis in an inguinal hernia mouse model.

JCI insight

Potluri T,Taylor MJ,Stulberg JJ,Lieber RL,Zhao H,Bulun SE

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Apr 19 00:00:00 EDT 2022

Phosphatase SHP1 impedes mesenchymal stromal cell immunosuppressive capacity modulated by JAK1/STAT3 and P38 signals.

Cell & bioscience

Jiang M,Ye J,Wang X,Li N,Wang Y,Shi Y

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Thu Apr 14 00:00:00 EDT 2022

Placental endocrine function shapes cerebellar development and social behavior.

Nature neuroscience

Vacher CM,Lacaille H,O'Reilly JJ,Salzbank J,Bakalar D,Sebaoui S,Liere P,Clarkson-Paredes C,Sasaki T,Sathyanesan A,Kratimenos P,Ellegood J,Lerch JP,Imamura Y,Popratiloff A,Hashimoto-Torii K,Gallo V,Schumacher M,Penn AA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

Extracellular vesicles synchronize cellular phenotypes of differentiating cells.

Journal of extracellular vesicles

Minakawa T,Matoba T,Ishidate F,Fujiwara TK,Takehana S,Tabata Y,Yamashita JK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Extracellular vesicles synchronize cellular phenotypes of differentiating cells.

Journal of extracellular vesicles

Minakawa T,Matoba T,Ishidate F,Fujiwara TK,Takehana S,Tabata Y,Yamashita JK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Immunocytochemistry

Wed Sep 01 00:00:00 EDT 2021

Mesothelial cells are not a source of adipocytes in mice.

Cell reports

Westcott GP,Emont MP,Li J,Jacobs C,Tsai L,Rosen ED

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Jul 13 00:00:00 EDT 2021

Zbtb16 regulates social cognitive behaviors and neocortical development.

Translational psychiatry

Usui N,Berto S,Konishi A,Kondo M,Konopka G,Matsuzaki H,Shimada S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Immunohistochemistry

Sat Apr 24 00:00:00 EDT 2021

Interleukin-11-expressing fibroblasts have a unique gene signature correlated with poor prognosis of colorectal cancer.

Nature communications

Nishina T,Deguchi Y,Ohshima D,Takeda W,Ohtsuka M,Shichino S,Ueha S,Yamazaki S,Kawauchi M,Nakamura E,Nishiyama C,Kojima Y,Adachi-Akahane S,Hasegawa M,Nakayama M,Oshima M,Yagita H,Shibuya K,Mikami T,Inohara N,Matsushima K,Tada N,Nakano H

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Fri Apr 16 00:00:00 EDT 2021

IGF1R controls mechanosignaling in myofibroblasts required for pulmonary alveologenesis.

JCI insight

He H,Snowball J,Sun F,Na CL,Whitsett JA

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

Gain-of-function p53 protein transferred via small extracellular vesicles promotes conversion of fibroblasts to a cancer-associated phenotype.

Cell reports

Ma S,McGuire MH,Mangala LS,Lee S,Stur E,Hu W,Bayraktar E,Villar-Prados A,Ivan C,Wu SY,Yokoi A,Dasari SK,Jennings NB,Liu J,Lopez-Berestein G,Ram P,Sood AK

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2021

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells.

PloS one

Falero-Perez J,Sheibani N,Sorenson CM

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Fri Jul 31 00:00:00 EDT 2020

Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice.

Nature communications

Wang S,Cao S,Arhatte M,Li D,Shi Y,Kurz S,Hu J,Wang L,Shao J,Atzberger A,Wang Z,Wang C,Zang W,Fleming I,Wettschureck N,Honoré E,Offermanns S

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Fri May 08 00:00:00 EDT 2020

Tissue-Resident PDGFRα⁺ Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner.

Cell reports

Santini MP,Malide D,Hoffman G,Pandey G,D'Escamard V,Nomura-Kitabayashi A,Rovira I,Kataoka H,Ochando J,Harvey RP,Finkel T,Kovacic JC

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Hyaluronan Degradation by Cemip Regulates Host Defense against Staphylococcus aureus Skin Infection.

Cell reports

Dokoshi T,Zhang LJ,Li F,Nakatsuji T,Butcher A,Yoshida H,Shimoda M,Okada Y,Gallo RL

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

Dual lineage tracing identifies intermediate mesenchymal stage for endocardial contribution to fibroblasts, coronary mural cells, and adipocytes.

The Journal of biological chemistry

Huang X,Feng T,Jiang Z,Meng J,Kou S,Lu Z,Chen W,Lin CP,Zhou B,Zhang H

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Fri May 31 00:00:00 EDT 2019

A Brain-Melanocortin-Vagus Axis Mediates Adipose Tissue Expansion Independently of Energy Intake.

Cell reports

Holland J,Sorrell J,Yates E,Smith K,Arbabi S,Arnold M,Rivir M,Morano R,Chen J,Zhang X,Dimarchi R,Woods SC,Sanchez-Gurmaches J,Wohleb E,Perez-Tilve D

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue May 21 00:00:00 EDT 2019

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Mar 05 00:00:00 EST 2019

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Tue Dec 11 00:00:00 EST 2018

The Isl1/Ldb1 Complex Orchestrates Genome-wide Chromatin Organization to Instruct Differentiation of Multipotent Cardiac Progenitors.

Cell stem cell

Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

Published figure using CD140a (PDGFRA) monoclonal antibody (Product # 61-1401-82) in Flow Cytometry

Thu Sep 03 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; MGC74795; OTTHUMP00000218656; PDGF A-chain; PDGF alpha chain; PDGF Receptor alpha; PDGF-R-alpha; pdgfr-a; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha

Gene Aliases: AI115593; CD140a; Pdgfr-2; Pdgfra

UniProt ID: (Mouse) P26618

Entrez Gene ID: (Mouse) 18595

Molecular Function: transmembrane signal receptor