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Invitrogen

CD140a (PDGFRA) Monoclonal Antibody (APA5), PE-eFluor 610, eBioscience™

View all (58) PDGFRA (CD140a) antibodies
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Product Details

Tested Applications
Dilution
Flow Cytometry (Flow)
0.25 µg/test
Product Specifications
Species Reactivity
Mouse
Host / Isotype
Rat / IgG2a, kappa
Recommended Isotype Control
Rat IgG2a kappa Isotype Control (eBR2a), PE-eFluor 610, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
APA5
Conjugate
PE-eFluor® 610
Excitation/Emission Profile
View spectra
Form
Liquid
Concentration
0.2 mg/ml
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!

Product Specific Information

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.

Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red).  Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

Target Information

PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a; PDGF alpha chain; PDGF-R-alpha; PDGFR-alpha; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor alpha

Gene Aliases: AI115593; CD140a; Pdgfr-2; Pdgfra

UniProt ID: (Mouse) P26618

Entrez Gene ID: (Mouse) 18595

Function(s)
luteinization in utero embryonic development hematopoietic progenitor cell differentiation protein phosphorylation chemotaxis transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cytosolic calcium ion concentration multicellular organism development estrogen metabolic process positive regulation of cell proliferation female gonad development anatomical structure morphogenesis organ morphogenesis negative regulation of platelet activation positive regulation of phospholipase C activity phosphorylation cell migration peptidyl-tyrosine phosphorylation signal transduction involved in regulation of gene expression extracellular matrix organization lung development adrenal gland development positive regulation of cell migration male genitalia development Leydig cell differentiation cellular response to reactive oxygen species platelet-derived growth factor receptor-alpha signaling pathway cellular response to platelet-derived growth factor stimulus positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway wound healing odontogenesis of dentin-containing tooth positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication protein autophosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis skeletal system morphogenesis regulation of chemotaxis cardiac myofibril assembly palate development face morphogenesis cell chemotaxis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade platelet aggregation cellular response to amino acid stimulus metanephric glomerular capillary formation regulation of mesenchymal stem cell differentiation
Process(es)
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity platelet-derived growth factor receptor binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol 3-kinase binding platelet-derived growth factor binding

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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