Invitrogen

CD16 Monoclonal Antibody (ASH 1975)

Product Image Gallery

CD16 Antibody in Immunohistochemistry (IHC) — Figure 3 Modulation of microglia phenotype and the potential mechanism of action by Ma@(MnO 2 +FTY) nanoparticles. a) Expression of P65 in the cytoplasm and nucleus of microglia with different treatment, characterized by western blotting. b) Representative images of p-P65 in microglia with different treatment which attenuated the expression of p-P65 and reduced the transfer to the nucleus (scale bar, 25 um). c) Expression of STAT3-associated protein in microglia with different treatment. d) Scheme of the mechanism for microglia polarization by Ma@(MnO 2 +FTY) nanoparticles. e) Representative images of microglia immunostained with CD16/32 and CD206 (scale bar, 100 um). f) Quantification of the relative fluorescence intensity of CD206 versus CD16/32. Data are presented as means +- SD, n = 3, ** P < 0.01.

Product Details

MA1-7633

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	1:50	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	1:100	-
ELISA (ELISA)	1:1,000	-
Immunoprecipitation (IP)	1:50	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	ASH 1975
Immunogen	CD16, Human white blood cell fraction View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.2
Contains	0.01% sodium azide
Storage conditions	4°C short term, -80°C long term
RRID	AB_2103889

Product Specific Information

MA1-7633 detects CD16 from human samples and CD16-2 from mouse and rat.

MA1-7633 has been successfully used in ELISA, IF, flow cytometry, immunoprecipitation, and immunohistochemistry (frozen sections) procedures.

The MA1-7633 immunogen is a CD16 protein.

Store at 4°C short term. For extended storage, store at -80°C.

Target Information

CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Macrophage-Disguised Manganese Dioxide Nanoparticles for Neuroprotection by Reducing Oxidative Stress and Modulating Inflammatory Microenvironment in Acute Ischemic Stroke.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Li C,Zhao Z,Luo Y,Ning T,Liu P,Chen Q,Chu Y,Guo Q,Zhang Y,Zhou W,Chen H,Zhou Z,Wang Y,Su B,You H,Zhang T,Li X,Song H,Li C,Sun T,Jiang C

Published figure using CD16 monoclonal antibody (Product # MA1-7633) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

Microglia Demonstrate Local Mixed Inflammation and a Defined Morphological Shift in an APP/PS1 Mouse Model.

Journal of Alzheimer's disease : JAD

Holloway OG,King AE,Ziebell JM

MA1-7633 was used in Immunohistochemistry to indicate that pro- and anti-inflammatory factors of microglia occur in APP/PS1 mice.

Fri Sep 10 00:00:00 EDT 2021

Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH.

Nature protocols

Messal HA,Almagro J,Zaw Thin M,Tedeschi A,Ciccarelli A,Blackie L,Anderson KI,Miguel-Aliaga I,van Rheenen J,Behrens A

MA1-7633 was used in Immunocytochemistry to describe FLASH (fast light-microscopic analysis of antibody-stained whole organs), a simple, rapid, fully customizable technique for molecular phenotyping of intact tissue volumes.

Fri Jan 01 00:00:00 EST 2021

Bioinformatics

Protein Aliases: CD16-2; CD16a; CD16a antigen; CD16b; CD32 receptor 2; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc gamma receptor III; Fc gamma receptor III-A; Fc gamma receptor IIIa; Fc gamma receptor IIIb; Fc gammaRIV; Fc receptor-like 3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIII-beta; Fc-gamma RIIIa; fc-gamma RIIIb; Fcg receptor III; FCG2; FcgammaRIII; FcgammaRIIIA; FcgammaRIV; FCGR2; FCGR2A; FCGR2B; FCGR2C; FcR-10; FcRII-b; FcRII-c; fcRIII; igG Fc receptor III; IgG Fc receptor III-1; IgG Fc receptor III-2; immunoglobulin G Fc receptor III; low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Low affinity immunoglobulin gamma Fc region receptor III-B; Low affinity immunoglobulin gamma Fc region receptor IV; neutrophil-specific antigen NA; RP11-5K23.1

Gene Aliases: 4833442P21Rik; CD16; CD16-2; CD16A; CD16B; FCG3; FcgammaRIV; FCGR3; FCGR3A; FCGR3B; Fcgr4; FCGRIII; FcgRIV; FCR-10; FCRIII; FCRIIIA; FCRIIIb; Fcrl3; IGFR3; IMD20

UniProt ID: (Human) P08637, (Human) O75015, (Mouse) A0A0B4J1G0

Entrez Gene ID: (Human) 2214, (Human) 2215, (Mouse) 14131, (Mouse) 246256

Molecular Function: immunoglobulin receptor superfamily