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Invitrogen

CD202b (TIE2) Monoclonal Antibody (TEK4), PE, eBioscience™

10 Published Figures
30 References
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Product Details

Tested Applications
Dilution
Flow Cytometry (Flow)
2 µg/test
Published Applications
Flow Cytometry (Flow)
See 27 publications below
Immunofluorescence (IF)
See 1 publication below
Immunohistochemistry (IHC)
See 1 publication below
Immunohistochemistry (Frozen) (IHC (F))
See 1 publication below
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host / Isotype
Rat / IgG1, kappa
Recommended Isotype Control
Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
TEK4
Conjugate
PE
Excitation/Emission Profile
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% gelatin
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_466100

Product Specific Information

Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.

Applications Reported: The TEK4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The TEK4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: angiopoietin-1 receptor; CD202B; endothelial tyrosine kinase; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TIE-2; TIE2; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protei; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2; VMCM; VMCM1

Gene Aliases: AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2

Entrez Gene ID: (Mouse) 21687

Function(s)
angiogenesis patterning of blood vessels vasculogenesis positive regulation of protein phosphorylation endothelial cell proliferation regulation of endothelial cell proliferation sprouting angiogenesis positive regulation of cytokine secretion involved in immune response protein phosphorylation cell-matrix adhesion transmembrane receptor protein tyrosine kinase signaling pathway heart development positive regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling phosphorylation single organismal cell-cell adhesion negative regulation of angiogenesis peptidyl-tyrosine phosphorylation hemopoiesis regulation of cell migration positive regulation of vascular endothelial growth factor receptor signaling pathway response to retinoic acid regulation of establishment or maintenance of cell polarity positive regulation of peptidyl-serine phosphorylation substrate adhesion-dependent cell spreading intracellular signal transduction positive regulation of protein import into nucleus negative regulation of apoptotic process positive regulation of phosphatidylinositol 3-kinase activity response to estrogen regulation of angiogenesis positive regulation of angiogenesis positive regulation of cell adhesion protein autophosphorylation Tie signaling pathway protein oligomerization positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling heart trabecula formation positive regulation of ERK1 and ERK2 cascade regulation of NIK/NF-kappaB signaling positive regulation of intracellular signal transduction positive regulation of actin cytoskeleton reorganization negative regulation of endothelial cell apoptotic process
Process(es)
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding

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