Invitrogen

CD249 (BP-1) Monoclonal Antibody (6C3), PE, eBioscience™

1 Published Figure

16 References

View all (38) CD249 (BP-1) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD249 (BP-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD249 (BP-1) Antibody (12-5891-82) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.5 µg of Anti-Mouse BP-1 PE (right). Samples were pre-incubated with Anti-Mouse CD16/CD32 Purified (Product # 14-0161-82). Total viable cells were used for analysis.

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Product Details

12-5891-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6C3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466015

Product Specific Information

Description: The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.^M
^M
Applications Reported: The 6C3 antibody has been reported for use in flow cytometric analysis.^M
^M
Applications Tested: The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.^M
^M
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.^M
^M
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD249 appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Embryonic keratin19⁺ progenitors generate multiple functionally distinct progeny to maintain epithelial diversity in the adult thymus medulla.

Nature communications

Lucas B,White AJ,Klein F,Veiga-Villauriz C,Handel A,Bacon A,Cosway EJ,James KD,Parnell SM,Ohigashi I,Takahama Y,Jenkinson WE,Hollander GA,Lu WY,Anderson G

Published figure using CD249 (BP-1) monoclonal antibody (Product # 12-5891-82) in Flow Cytometry

Wed Apr 12 00:00:00 UTC 2023

LAMP2 regulates autophagy in the thymic epithelium and thymic stroma-dependent CD4 T cell development.

Autophagy

Rodrigues PM,Sousa LG,Perrod C,Maceiras AR,Ferreirinha P,Pombinho R,Romera-Cárdenas G,Gomez-Lazaro M,Senkara M,Pistolic J,Cabanes D,Klein L,Saftig P,Alves NL

12-5891-82 was used in Flow Cytometry to suggest that LAMP2 interconnects the autophagy-lysosomal axis and the processing of selecting self-peptides:MHC II complexes in cTECs, underling its implications for the generation of a broad CD4 TCR repertoire.

Wed Feb 01 00:00:00 UTC 2023

Lymphocyte-Specific Function of the DNA Polymerase Epsilon Subunit Pole3 Revealed by Neomorphic Alleles.

Cell reports

Siamishi I,Iwanami N,Clapes T,Trompouki E,O'Meara CP,Boehm T

12-5891 was used in Flow cytometry/Cell sorting to serve as a paradigm to understand the molecular basis of cell-type-specific non-replicative functions of the ubiquitous POLE complex.

Tue Jun 16 00:00:00 UTC 2020

Epigenetic Protection of Vertebrate Lymphoid Progenitor Cells by Dnmt1.

iScience

Iwanami N,Takeshita K,Lawir DF,Suetake I,Tajima S,Sikora K,Trancoso I,ÓMeara C,Siamishi I,Takahama Y,Furutani-Seiki M,Kondoh H,Yonezawa Y,Schorpp M,Boehm T

12-5891 was used in Flow cytometry/Cell sorting to identify new recessive dnmt1 alleles in medaka and zebrafish and, guided by the structures of mutant proteins, generated a recessive variant of mouse Dnmt1.

Fri Jul 24 00:00:00 UTC 2020

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

12-5891-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 UTC 2019

Fundamental parameters of the developing thymic epithelium in the mouse.

Scientific reports

Hirakawa M,Nagakubo D,Kanzler B,Avilov S,Krauth B,Happe C,Swann JB,Nusser A,Boehm T

12-5891 was used in Flow cytometry/Cell sorting to study the dynamic of thymic epithelial cells and thymocytes during embryogenesis.

Mon Jul 23 00:00:00 UTC 2018

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD249 (BP-1) monoclonal antibody (Product # 12-5891-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2016

Distinctive Mesenchymal-Parenchymal Cell Pairings Govern B Cell Differentiation in the Bone Marrow.

Stem cell reports

Yu VW,Lymperi S,Oki T,Jones A,Swiatek P,Vasic R,Ferraro F,Scadden DT

12-5891 was used in Flow cytometry/Cell sorting to study the notion that bone marrow is a composite of specialised niches, providing distinctive functional units to regulate hematopoiesis.

Tue Aug 09 00:00:00 UTC 2016

Mutations in G protein β subunits promote transformation and kinase inhibitor resistance.

Nature medicine

Yoda A,Adelmant G,Tamburini J,Chapuy B,Shindoh N,Yoda Y,Weigert O,Kopp N,Wu SC,Kim SS,Liu H,Tivey T,Christie AL,Elpek KG,Card J,Gritsman K,Gotlib J,Deininger MW,Makishima H,Turley SJ,Javidi-Sharifi N,Maciejewski JP,Jaiswal S,Ebert BL,Rodig SJ,Tyner JW,Marto JA,Weinstock DM,Lane AA

12589182 was used in flow cytometry to investigate the role of GNB1 and GNB2 in cancer cells.

Thu Jan 01 00:00:00 UTC 2015

Premature thymic involution is independent of structural plasticity of the thymic stroma.

European journal of immunology

Franckaert D,Schlenner SM,Heirman N,Gill J,Skogberg G,Ekwall O,Put K,Linterman MA,Dooley J,Liston A

12-5891 was used in Flow cytometry/Cell sorting to investigate thymic involution in FVB/N mice, showing that disruption of the epithelial-endothelial relationship and a progressive loss of pro-T cells precedes involution, and loss of pro-T cells is sufficient to drive premature involution, suggesting that pro-T cells are the main driver of involution.

Fri May 01 00:00:00 UTC 2015

Osteoprotegerin-Mediated Homeostasis of Rank+ Thymic Epithelial Cells Does Not Limit Foxp3+ Regulatory T Cell Development.

Journal of immunology (Baltimore, Md. : 1950)

McCarthy NI,Cowan JE,Nakamura K,Bacon A,Baik S,White AJ,Parnell SM,Jenkinson EJ,Jenkinson WE,Anderson G

12-5891 was used in Flow cytometry/Cell sorting to elucidate the conditions controlling medullary thymic epithelial cell (mTEC) homeostasis and the production of mTEC-dependent Foxp3(+) Tregs.

Tue Sep 15 00:00:00 UTC 2015

Triplication of a 21q22 region contributes to B cell transformation through HMGN1 overexpression and loss of histone H3 Lys27 trimethylation.

Nature genetics

Lane AA,Chapuy B,Lin CY,Tivey T,Li H,Townsend EC,van Bodegom D,Day TA,Wu SC,Liu H,Yoda A,Alexe G,Schinzel AC,Sullivan TJ,Malinge S,Taylor JE,Stegmaier K,Jaffe JD,Bustin M,te Kronnie G,Izraeli S,Harris MH,Stevenson KE,Neuberg D,Silverman LB,Sallan SE,Bradner JE,Hahn WC,Crispino JD,Pellman D,Weinstock DM

12-5891 was used in Flow cytometry/Cell sorting to show that germline triplication of only 31 genes orthologous to human chromosome 21q22 confers mouse progenitor B cell self renew in vitro.

Sun Jun 01 00:00:00 UTC 2014

An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

White AJ,Jenkinson WE,Cowan JE,Parnell SM,Bacon A,Jones ND,Jenkinson EJ,Anderson G

12-5891 was used in Flow cytometry/Cell sorting to investigate whether thymus microenvironments are required in iNKT cell development, showing that medullary thymic epithelial cells are essential for intrathymic development of iNKT cells.

Sat Mar 15 00:00:00 UTC 2014

Phosphatase Wip1 is essential for the maturation and homeostasis of medullary thymic epithelial cells in mice.

Journal of immunology (Baltimore, Md. : 1950)

Sun L,Li H,Luo H,Zhang L,Hu X,Yang T,Sun C,Chen H,Zhang L,Zhao Y

12-5891 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms controlling thymic epithelial cell differentiation and activities.

Sun Sep 15 00:00:00 UTC 2013

Developmentally regulated availability of RANKL and CD40 ligand reveals distinct mechanisms of fetal and adult cross-talk in the thymus medulla.

Journal of immunology (Baltimore, Md. : 1950)

Desanti GE,Cowan JE,Baik S,Parnell SM,White AJ,Penninger JM,Lane PJ,Jenkinson EJ,Jenkinson WE,Anderson G

12-5891 was used in Flow cytometry/Cell sorting to study how multiple hemopoietic cell types regulate mTEC development through differential provision of RANKL/CD40L during ontogeny, revealing molecular differences in foetal and adult hemopoietic cross-talk.

Sat Dec 15 00:00:00 UTC 2012

Frizzled 9 knock-out mice have abnormal B-cell development.

Blood

Ranheim EA,Kwan HC,Reya T,Wang YK,Weissman IL,Francke U

12-5891 was used in Flow cytometry/Cell sorting to suggest a role for frizzled 9 signalling in lymphoid development.

Tue Mar 15 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: Aminopeptidase A; aminopeptidase-A; AP-A; BP-1/6C3 antigen; BP1; CD249; EAP; Glutamyl aminopeptidase

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Gene Aliases: 6030431M22Rik; APA; Bp-1/6C3; Enpep; Ly-51; Ly51

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UniProt ID: (Mouse) P16406

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Entrez Gene ID: (Mouse) 13809

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Function(s)

aminopeptidase activity peptidase activity metallopeptidase activity zinc ion binding hydrolase activity peptide binding metal ion binding metalloaminopeptidase activity metalloprotease

Process(es)

angiogenesis regulation of systemic arterial blood pressure by renin-angiotensin proteolysis cellular aromatic compound metabolic process cell proliferation cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD249 (BP-1) Monoclonal Antibody (6C3), PE, eBioscience™

CD249 (BP-1) Antibody (12-5891-82) in Flow

Product Details

12-5891-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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