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Invitrogen

CD309 (FLK1) Monoclonal Antibody (Avas12a1), PE, eBioscience™

26 Published Figures
34 References
View all (49) FLK1 (VEGF Receptor 2) antibodies
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Product Details

Tested Applications
Dilution
Flow Cytometry (Flow)
0.5 µg/test
Published Applications
Flow Cytometry (Flow)
See 34 publications below
Product Specifications
Species Reactivity
Mouse
Published species
Human, Mouse
Host / Isotype
Rat / IgG2a, kappa
Recommended Isotype Control
Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
Avas12a1
Conjugate
PE
Excitation/Emission Profile
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.1% gelatin
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_465967

Product Specific Information

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported: The Avas12a1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 6130401C07; fetal liver kinase 1; Flk-1; Flk1; kinase NYK; Krd-1; Ly73; protein-tyrosine kinase receptor flk-1; soluble vascular endothelial growth factor receptor 2; sVEGFR-2; vascular endothelial growth factor receptor 2; vascular endothelial growth factor receptor- 2; vascular endothelial growth factor receptor-2; vascular endothelial growth factor receptor-3; VEGF receptor-2; VEGFR2

Gene Aliases: 6130401C07; Flk-1; Flk1; Krd-1; Ly73; sVEGFR-2; VEGFR-2; VEGFR2

Entrez Gene ID: (Mouse) 16542

Function(s)
angiogenesis ovarian follicle development vasculogenesis response to hypoxia positive regulation of protein phosphorylation regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation lymph vessel development cell migration involved in sprouting angiogenesis positive regulation of mesenchymal cell proliferation endocardium development endothelium development endochondral bone growth protein phosphorylation transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cytosolic calcium ion concentration multicellular organism development positive regulation of cell proliferation regulation of cell shape male gonad development positive regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling phosphorylation cell migration peptidyl-tyrosine phosphorylation hemopoiesis cell differentiation lung development positive regulation of cell migration positive regulation of BMP signaling pathway positive regulation of vascular endothelial growth factor receptor signaling pathway positive regulation of TOR signaling embryonic hemopoiesis calcium-mediated signaling using intracellular calcium source cellular response to vascular endothelial growth factor stimulus peptidyl-tyrosine autophosphorylation vascular endothelial growth factor signaling pathway response to drug negative regulation of apoptotic process surfactant homeostasis positive regulation of MAPK cascade negative regulation of neuron apoptotic process cell fate commitment endothelial cell differentiation regulation of endothelial cell differentiation positive regulation of angiogenesis positive regulation of vasodilation protein autophosphorylation vascular endothelial growth factor receptor signaling pathway positive regulation of long-term neuronal synaptic plasticity lung alveolus development cell maturation branching morphogenesis of an epithelial tube neuron projection morphogenesis positive regulation of epithelial cell proliferation positive regulation of calcium-mediated signaling positive regulation of positive chemotaxis positive regulation of nitric-oxide synthase biosynthetic process positive regulation of focal adhesion assembly calcium ion homeostasis blood vessel endothelial cell differentiation positive regulation of ERK1 and ERK2 cascade regulation of hematopoietic progenitor cell differentiation negative regulation of endothelial cell apoptotic process positive regulation of vasculogenesis
Process(es)
nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity vascular endothelial growth factor-activated receptor activity integrin binding protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding vascular endothelial growth factor binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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